関連する実験動画
Updated: Jul 11, 2026
14:57
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
p53のC端末ドメインのアセチル化により,p53配列特異のDNA結合の活性化
1Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York 10021, USA.
Cell
|August 22, 1997
まとめ
腫瘍を抑制するp53タンパク質は,
科学分野:
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
- 遺伝学 遺伝学とは
背景:
- 腫瘍抑制剤p53は,細胞増殖を抑制するために重要な転写因子です.
- p53の抗増殖機能は,そのDNA結合活動に依存しています.
- p53のDNA結合の調節は,その腫瘍抑制作用にとって極めて重要です.
研究 の 目的:
- アセチル化によるp53の翻訳後の改変を調査する.
- DNA結合活性に対するp53アセチル化の機能的影響を決定する.
- 転写共同活性化剤の文脈におけるp53アセチル化の役割を調査する.
主な方法:
- p53.53のインビヴォおよびインビトロアセチル化アッセイ.
- p53の特定のアセチル化部位の識別.
- アセチル化後のp53のDNA結合活性の分析.
主要な成果:
- p53は,in vivoとin vitroの両方でアセチル化されます.
- アセチル化は,p53のDNA結合に不可欠なC端末ドメインで起こります.
- p300によるアセチル化は,p53の配列特異的なDNA結合活性を大幅に高めます.
- アセチル化により,p53の形状の変化が起こり,その機能が調節される可能性があります.
結論:
- アセチル化は,p53機能を活性化するための新しいメカニズムを表しています.
- この研究は,非ヒストン調節タンパク質におけるアセチル化媒介による機能的変化の証拠を提供します.
- この発見は,ヒストンの改変を超えてアセチルトランスフェラーゼ共同活性化剤のメカニズムを理解するための意味を持つ.
関連する概念動画
02:04Covalently Linked Protein Regulators
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
02:36DNA Damage can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
02:23Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
02:36DNA Damage Can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...