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Updated: Aug 3, 2026
07:44
High-throughput Purification of Affinity-tagged Recombinant Proteins
Published on: August 26, 2012
酵母TAF (II) 145は,コアプロモーターの選択性因子として機能するが,一般的な共同活性化因子ではない
1Howard Hughes Medical Institute, Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester 01605, USA.
Cell
|August 22, 1997
まとめ
TATAボックス結合タンパク質関連因子 (TAFII) は,通常,酵母遺伝子の転写に不可欠ではありません. しかし,コアプロモーターで機能する遺伝子のサブセットには,特定の因子,yTAF ((II) 145) が必要です.
科学分野:
- 分子生物学は分子生物学である.
- イースト遺伝学 イースト遺伝学
- 遺伝子規制 遺伝子規制
背景:
- TATAボックス結合タンパク質関連因子 (TAFII) は,一般的にほとんどの酵母遺伝子転写に欠かせないと考えられています.
- 遺伝子調節における個々のTAF (II) の特定の役割は,まだ完全に理解されていません.
研究 の 目的:
- TATAボックス結合タンパク質関連因子 yTAF ((II) 145) を必要とする酵母遺伝子を in vivo 転写のために識別する.
- yTAF ((II)) 145依存転写に責任のあるプロモーター領域を明らかにする.
主な方法:
- yTAF(II) 145依存遺伝子を識別するために,差異表示が使用されました.
- 重要なDNA領域を正確に特定するために,プロモーターマッピング研究が行われました.
- トランスクリプションアッセイは,様々なアップストリームアクティベーション配列とアクティベーターの欠如で実施されました.
主要な成果:
- 酵母遺伝子の小さなサブセットは,転写のためにyTAF (II) 145を必要としていると特定されました.
- アップストリームアクティベーションシーケンスではなく,コアプロモーターは,yTAF (II) 145依存を決定する領域であることが判明しました.
- この yTAF ((II) 145) の要求は,異なる上流のアクティベーションシーケンスやアクティベーターがない場合でも維持された.
結論:
- yTAF ((II) 145) は,特定の酵母遺伝子の転写に重要な役割を果たしています.
- yTAFの機能は,コアプロモーターの認識と選択を伴う.
- このメカニズムは,上流の活性化剤結合部位とは独立して動作する.
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