Wntシグナリングは,初期のC. elegansブラストオメアを二極化して,内皮と中皮を区別する
C J Thorpe1, A Schlesinger, J C Carter
1Institute of Molecular Biology, University of Oregon, Eugene 97403, USA.
Cell
|August 22, 1997
まとめ
5つの遺伝子 (MOM-1からMOM-5) は,C. elegans.の内皮の発達に不可欠です. このWnt信号伝達経路は,初期の胚芽細胞の細胞極性および遺伝子発現を調節する.
科学分野:
- 発達生物学 発達生物学とは
- 細胞生物学 細胞生物学
- 遺伝学 遺伝学とは
背景:
- C. elegans の 4 細胞段階の偏振信号は,EMS ブラストオメアをエンドードルマを生成するように指示します.
- この非対称な細胞運命を決定する分子機構は完全に理解されていません.
研究 の 目的:
- C. elegans. のEMSブラストメアによる内皮生殖に不可欠な遺伝子を特定する.
- 初期胚形成における細胞の極性形成におけるシグナル伝達経路の役割を明らかにする.
主な方法:
- 遺伝子スクリーニングを進めて,内皮の発達に影響を与える変異を特定します.
- 特定のブラストメア (P2シグナル伝達細胞およびEMS) の遺伝子機能の分析.
- 変異性胚におけるミトス・スパインドルの指向の検査.
主要な成果:
- EMS誘発の内皮生殖に必要な5つの遺伝子 (mom-1からmom-5) で16の変異を特定しました.
- mom-1, mom-2, mom-3はP2シグナル伝達細胞で機能し,mom-4はEMSブラストメアで作用する.
- P2シグナリングは,EMSの娘細胞のHMGドメインタンパク質POP-1をダウンレギュレーションします.
- mom-2はWntファミリーのメンバーをコードし,保存されたシグナル伝達経路を示唆しています.
- 変異はミトスのスパインドルの指向を乱し,初期のブラストメアにおける細胞骨格の極性におけるWnt信号伝達の役割を示している.
結論:
- Wnt経路のコンポーネントを含むMOM遺伝子は,C. elegans.における非対称性のある内皮特異性にとって重要である.
- P2派生信号によって媒介されるWntシグナリングは,EMS系統におけるPOP-1レベルと細胞骨格の極性に影響を与える.
- この経路は,胚の発達初期に複数のブラストメアにおける細胞の極性に影響を与えます.
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