ステロイド受容体コアクティベーター-1はヒストンアセチルトランスフェラーゼです
T E Spencer1, G Jenster, M M Burcin
1Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
Nature
|September 20, 1997
まとめ
ステロイド受容体共同活性化剤SRC-1はヒストンアセチルトランスフェラーゼ活性を持ち,遺伝子転写を助けます. この活動は,PCAFとともに,染色体抑制を克服し,遺伝子活性化を可能にします.
科学分野:
- 分子生物学は分子生物学である.
- エピジェネティクス エピジェネティクス
- 遺伝子規制 遺伝子規制
背景:
- ステロイド受容体とコアクティベーターは,通常,転写複合体を形成することによって,遺伝子発現を強化します.
- 抑制されたクロマチンのアクセスは, in vivo の遺伝子活性化を理解する上で重要な問題です.
- ヒストンアセチルトランスフェラーゼ (HATs) は,クロマチンの転写に対する抑制効果に対抗することが知られている.
研究 の 目的:
- 染色体調節におけるステロイド受容体共活性化剤SRC-1の役割を調査する.
- SRC-1が固有ヒストンアセチルトランスファーゼ (HAT) 活性を持っているかどうかを判断する.
- 遺伝子転写におけるSRC-1,PCAF,ヒストンのアセチル化との相互作用を探求する.
主な方法:
- SRC-1のHAT活性を評価するための生化学的測定法.
- SRC-1とPCAFの結合を検証するタンパク質相互作用の研究.
- 特定の遺伝子プロモーターのヒストンアセチル化パターンの分析.
主要な成果:
- SRC-1はHATの内在的な活性を示し,主にヒストーンH3とH4を標的とする.
- SRC-1は,別のHATであるp300/CBP関連因子 (PCAF) と相互作用する.
- ステロイド受容体へのリガンド結合は,SRC-1およびPCAF媒介ヒストンアセチル化を誘発する可能性があります.
結論:
- SRC-1のHAT活動は,染色体抑制を克服するために重要である.
- SRC-1とPCAFの協調した作用により,転写因子の抑制されたクロマチンへのアクセスを促進します.
- このメカニズムは,ステロイド受容体媒介の遺伝子活性化を強化し,特定の遺伝子転写を増加させます.
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The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
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