エクスポートイン1 (Crm1p) は核の輸出に不可欠な要素である

K Stade¹, C S Ford, C Guthrie

¹Department of Biochemistry and Biophysics, University of California at San Francisco, 94143-0414, USA.

Cell

|October 10, 1997

まとめ

研究者らは,酵母における核輸出のための重要なタンパク質としてCRM1を特定した. この発見は,タンパク質とmRNAを細胞核から外に輸送するための重要なメカニズムを明らかにしています.

科学分野:

細胞生物学細胞生物学
分子生物学は分子生物学である.
遺伝学遺伝学とは

背景:

核タンパク質の輸出は,細胞の機能に不可欠である.
核輸出の正確なメカニズム,特に核輸出信号 (NES) を含むものは,まだ完全に理解されていません.
これらの経路を理解することは,細胞の輸送プロセスを解読する上で極めて重要です.

研究の目的:

酵母 (Saccharomyces cerevisiae) の核タンパク質輸出の主要な媒介者を特定する.
核輸送におけるCRM1の役割を明らかにする.
タンパク質とmRNAの輸出経路の関係を調査する.

主な方法:

S. cerevisiaeにおける新しいタンパク質輸出測定法の開発.
CRM1タンパク質とその相互作用の特徴.
Crm1pの変異とその核輸出への影響の分析.

主要な成果:

CRM1は,酵母における核タンパク質の輸出の重要な媒介者として特定されました.
Crm1pは,インポートリンベータ型輸送因子とのホモロジーを示した.
Crm1pは,NESモチーフとRan GTPaseの両方と特異的に相互作用する.
Crm1pの変異は,タンパク質とmRNAの輸出の両方を妨害し,結合された経路を示唆しました.

結論:

Crm1pは,NES媒介のタンパク質輸出経路のキャリアとして機能する.
核タンパク質の輸出とmRNAの輸出は,S. cerevisiae.で密接に結びついている.
研究では,CRM1をExportin 1 (XPO1) に改名することを提案しています.

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