S. cerevisiaeにおけるDNA複製複合体の構成要素とダイナミクス:S相中のMCMタンパク質とCdc45pの再分布
O M Aparicio1, D M Weinstein, S P Bell
1Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Cell
|October 23, 1997
まとめ
酵母では,ORC,Cdc45p,MCMなどの主要なタンパク質が,DNA複製の起源でプリレプリカーション複合体 (pre-RC) を形成する. これらのタンパク質は,S相の間に複製フォークで移動し,DNA複製のエントリーを調節します.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 遺伝学 遺伝学とは
背景:
- S. cerevisiaeのDNA複製におけるクロマチンの構造は,G1期中に変化する.
- これは,複製因子のG1相特異的関連が,起源DNAがS相への入り口を調節していることを示唆している.
研究 の 目的:
- プリレプリケーション複合体 (pre-RC) のタンパク質成分を特定する.
- G1/S 移行と S 段階における複製因子の振る舞いを特徴づける.
主な方法:
- クロマチン免疫プレシピテーションアッセイ.
- 細胞サイクル中の原産および非原産DNAとのタンパク質関連性の分析.
主要な成果:
- ORC,Cdc45p,MCMタンパク質は,RC前タンパク質の構成要素である.
- MCM起源の関連性は,ORCとCdc6pに依存しています.
- MCMタンパク質とCdc45pはS相において原産DNAから解離し,DNAポリメラーゼエプシロンに類似した非原産DNAと結合する.
結論:
- G1/Sトランジションにおけるプレ-RCと新複製複合体 (RC) のタンパク質成分を特定した.
- MCMタンパク質とCdc45pは,開始後,真核細胞の複製フォークで移動する可能性が高い.
関連する概念動画
03:01The Replisome
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
02:37Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
02:20Cohesins
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
03:01The Replisome
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.