GRASP65は,ゴルジの水槽の積み重ねに関与するタンパク質です
F A Barr1, M Puype, J Vandekerckhove
1Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Cell
|November 5, 1997
まとめ
N-エチルマレイミド (NEM) は,ゴルギの再組み立てスタッキングタンパク質 (GRASP65) を標的にし,ミトーシス中にゴルギの再組み立てを防止します. GRASP65とGM130のタンパク質は,酸化された場合でも,ゴルギ堆積と膀ドッキングに不可欠です.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- ゴルジ装置はミトーシス過程で断片化され,水槽が分散する.
- ミトーシス後のゴルギ・スタックの再組みは,細胞機能にとって不可欠です.
- ゴルギの構造と膀輸送に関与するタンパク質は,ミトスのゴルギの動態を理解するための重要な標的です.
研究 の 目的:
- N-エチルマレイミド (NEM) の分子標的を特定し,ミトーシス中にゴルギの再組み立てを阻害する.
- ゴルギのスタッキングにおける特定された標的タンパク質の役割とその他のゴルギのタンパク質との関係を特徴づける.
主な方法:
- NEMで細胞と細胞のないシステムを処理する.
- 抗体と再結合タンパク質を用いた抗体生成と機能的測定法.
- タンパク質複合体を研究するための共免疫プレシピテーション.
- ミトスの条件下でタンパク質のリン酸化の分析.
主要な成果:
- NEMは,ミトーシス後のゴルギの水槽の堆積構造への再組みを阻害する.
- Golgi ReAssembly Stacking Protein (GRASP65) と指定された65 kDaのタンパク質は,主要なNEM標的として特定されました.
- 抗体と再結合GRASP65は,Golgiの貯水池堆積を in vitroで阻害しました.
- 膀ドッキングに関与するGM130は,小規模なNEM標的として特定され,GRASP65.5と複合していることが判明しました.
- GRASP65とGM130はゴルギ膜に結合し,ミトーシス中にリン酸化される.
結論:
- GRASP65は,ゴルジ水槽の積み重ねには不可欠です.
- GRASP65-GM130複合体は,ゴルギ堆積と膀ドッキングの両方に役割を果たしています.
- ミトーシス中のGRASP65とGM130のリン酸化は,ゴルギの構造と機能の障害に寄与する可能性があります.
関連する概念動画
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Golgi Apparatus
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The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
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Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...


