プラズマウイルス血症の長期抑制にもかかわらず,複製能力のあるHIVの回復
J K Wong1, M Hezareh, H F Günthard
1Department of Medicine, University of California, San Diego, School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Science (New York, N.Y.)
|November 21, 1997
まとめ
効果的なHIV治療でも,潜伏ウイルスは患者から隔離することができます. この研究では,検出不能のプラズマHIVRNAにもかかわらず,細胞内の活性ウイルスが発見され,ウイルスの貯蔵庫が持続することを示しました.
科学分野:
- ウイルス学 ウイルス学 ウイルス学
- 免疫学 免疫学とは
- 感染症 感染症は感染症です.
背景:
- 現在のヒト免疫不全ウイルス (HIV) 併用薬療法は,ウイルス抑制を目的としています.
- 持続的なウイルス貯蔵庫を理解することは,HIVの根絶に不可欠です.
- 潜在的HIV貯蔵庫は,現在の治療戦略を回避することができます.
研究 の 目的:
- 併用薬物療法を受けている患者の潜在的HIV貯蔵体の存在を調査する.
- 長期のプラズマHIVRNA抑制にもかかわらず,ウイルスの再活性化が起こるかどうかを判断する.
主な方法:
- 周辺血液単核細胞 (PBMC) は,HIV患者および正常なドナーから採取されました.
- CD8細胞はPBMCから枯渇した.
- 患者のCD4リンパ球は,CD3およびCD28.2に対する固定された抗体を使用して活性化されました.
- PBMCからウイルスを分離した.
- HIVポリゲンの部分シーケンシングが行われました.
主要な成果:
- HIVは,プラズマHIVRNAが50コピー/mL未満の6人の患者のPBMCから2年間,成功裏に分離されました.
- HIVポリゲンの部分的な配列解析は,新しい薬剤耐性突然変異を明らかにしなかった.
- 配列化されたポリ遺伝子断片では,識別可能なウイルスの進化は観察されなかった.
結論:
- この発見は,持続的で潜伏するHIVの貯蔵庫の存在を証明する証拠を提供します.
- 薬の失敗ではなく,ウイルスの潜伏期が,PBMCからウイルスの分離を説明する.
- これらの潜伏貯水池は,HIVを完全に根絶するための課題となっています.
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