起源認識複合体のヘテロクロマチンとHP1との関連は,より高いエウカリオットで認められる
D T Pak1, M Pflumm, I Chesnokov
1Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
Cell
|November 18, 1997
まとめ
起源認識複合体 (ORC) は,ドロソフィラのヘテロクロマチンと結合し,HP1と相互作用する. これは,ORCが示唆している.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- エピジェネティクス エピジェネティクス
背景:
- 起源認識複合体 (ORC) は,真核生物におけるDNA複製を開始する.
- ORCはまた,酵母における転写静音化にも役割を果たしています.
- ヘテロクロマチンタンパク質1 (HP1) は,ポジションエフェクトバリエーション (PEV) に不可欠です.
研究 の 目的:
- ドロソフィラORC2とヘテロクロマチンとの関連を調査する.
- ORCとHPの相互作用を決定する1.
- ヘテロクロマチン組織におけるORCの役割を調査する.
主な方法:
- 染色体上のDmORC2とHP1の免疫ロケーション.
- バイオケミカルコイムノプレシピテーションアッセイ.
- DmORC2とPEVに影響する突然変異の分析.
主要な成果:
- ドロソフィラ ORC2は,インターフェーズとミトック染色体上のヘテロクロマチンと好ましく結合する.
- DmORCとHP1は,HP1のクロモドメインとシャドウドメインによって媒介される物理的な複合体を形成します.
- DmORC1のアミノ端はHP1結合部位を含んでいる.
- ヘテロジゴトのDmORC2変異はPEVを抑制する.
結論:
- ORCは,ヘテロクロマチン関連タンパク質HP1と相互作用する.
- ORCは,ヘテロクロマチン因子との相互作用によって染色体ドメインの包装に寄与する可能性があります.
- この相互作用は,DNA複製の開始を超えて,ゲノム組織におけるORCのより広範な役割を示唆しています.
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関連する概念動画
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Acetylation
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02:38Heterochromatin
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Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
01:01Euchromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
02:38Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...