ゲノムスケールでの遺伝子発現の代謝および遺伝的制御の探索
J L DeRisi1, V R Iyer, P O Brown
1Department of Biochemistry, Stanford University School of Medicine, Howard Hughes Medical Institute, Stanford, CA 94305-5428, USA.
Science (New York, N.Y.)
|October 24, 1997
まとめ
この研究では,発酵から呼吸への代謝シフト中のSaccharomyces cerevisiaeの遺伝子発現の変化をマッピングするためにDNAマイクロアレイを使用しました. それは,代謝の再プログラムと遺伝子機能の洞察を明らかにし,強力なゲノム全体のアプローチを示しました.
科学分野:
- 分子生物学は分子生物学である.
- イースト遺伝学 イースト遺伝学
- システム生物学 システム生物学
背景:
- メタボリックシフトを理解することは,酵母生物技術にとって極めて重要です.
- 遺伝子発現のダイナミクスは,細胞の適応を調節する.
- Saccharomyces cerevisiaeにおけるダイオキシ的シフトには,複雑な転写再プログラミングが伴う.
研究 の 目的:
- Saccharomyces cerevisiaeの発酵から呼吸への代謝シフト中の一時的な遺伝子発現プログラムを包括的に調査する.
- 代謝の再プログラムに関与する遺伝子を特定し,これまで特徴づけられなかった遺伝子の機能を明らかにする.
- トランスクリプションのレギュレータであるTUP1とYAP1がゲノム全体の遺伝子発現に与える影響を評価する.
主な方法:
- ほぼすべてのSaccharomyces cerevisiae遺伝子を代表するDNAマイクロアレイを使用しました.
- ダイオキシスシフト中のタイムラル遺伝子発現プロファイルを分析した.
- 調べられた遺伝子発現の変化は,TUP1の削除とYAP1.1の過剰発現によるものです.
主要な成果:
- 既知の代謝遺伝子の発現プロファイルは,ダイオキシスシフト中の代謝再プログラムに関する洞察を提供した.
- 特徴づけられていない遺伝子の発現パターンは,潜在的な機能を示唆した.
- TUP1の削除とYAP1の過剰発現によって影響を受ける遺伝子を特定しました.
結論:
- DNAマイクロアレイは,遺伝子発現パターンのゲノム全体の探査のための実行可能で強力なツールを提供します.
- この研究は,酵母における重要な代謝移行中の遺伝子発現ダイナミクスの詳細な地図を提供します.
- この発見は,酵母の代謝と遺伝子調節の理解に寄与する.
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