魚からのTc1型のトランポゾンである眠れる美女の分子再構築と,人間の細胞への移植
Z Ivics1, P B Hackett, R H Plasterk
1Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108-1095, USA.
Cell
|December 9, 1997
まとめ
研究者は,古代の魚の元素を再構築することによって,合成DNA-トランポソンシステムである睡眠美人トランポソンを設計しました. このアクティブシステムは,様々な脊椎動物の細胞における遺伝的変異と挿入性突然変異のための正確なカット&ペーストトランスポーゼーションを可能にします.
科学分野:
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
- 進化生物学の進化生物学について
背景:
- 魚のTc1/マリナートランスポーソンは,蓄積された変異により,典型的には不活性である.
- 古代のトランポゾン元素は,魚種間の水平遺伝子転送によって分散した可能性がある.
研究 の 目的:
- 不活性な魚の要素から機能するDNAトランポゾンシステムを再構築する.
- 脊椎動物における遺伝子操作のための活性トランポゾンを設計する.
主な方法:
- 分子系遺伝データを活用して,合成トランポゾン"眠れる美人"を設計した.
- 不活性化変異を除去することでコンセンサストランポゼゼ遺伝子を設計した.
- 魚,マウス,ヒトの細胞におけるスリーピング・ビューティのDNAトランポゾン活性を評価した.
主要な成果:
- エンジニアリングされたスリーピング・ビューティのトランポゼーゼは,トランポゾン逆転繰り返しに特異的に結合します.
- スリーピング・ビューティは,様々な脊椎動物の細胞タイプにおける正確なカット・アンド・ペースト変換を媒介する.
- スリーピング・ビューティが活性なDNAトランポゾンシステムであることを実証した.
結論:
- スリーピング・ビューティは,脊椎動物の機能的なDNA-トランポゾンシステムです.
- このシステムは,遺伝的変異と挿入性変異変生のために利用できます.
- 古代のトランポゾンを再構築することで,遺伝子研究のための新しいツールが提供されます.
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