関連する実験動画

Updated: Aug 11, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

ユカリオットRNAポリメラーゼによる核細胞を通しての転写のメカニズム

V M Studitsky1, G A Kassavetis, E P Geiduschek

  • 1Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

Science (New York, N.Y.)
|January 7, 1998
PubMed
まとめ

酵母RNAポリメラーゼIIIは,DNA包装の基本単位であるヌクレオソームを通して転写することができる. このプロセスは,直接のヒストン移転を含み,DNAの回転を指示する一時停止があり,異なるポリメラーゼで保存されたメカニズムを強調します.

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Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
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Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
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