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過酸化水素は,人間の大動脈内皮細胞における細胞内カルシウム振動を誘発する
1Department of Medicine, Johns Hopkins Bayview Medical Center, Johns Hopkins University School of Medicine, Baltimore, Md 21224-2780, USA.
Circulation
|February 14, 1998
まとめ
過酸化水素は,エンドプラズマ網膜の貯蔵物を放出することによって,ヒト内皮細胞の濃度依存カルシウム (Ca2+) 振動を引き起こします. これらの発見は,後発血性内皮機能不全を理解する上で極めて重要です.
科学分野:
- 細胞生物学 細胞生物学
- 生理学 生理学とは
- バイオケミストリー バイオケミストリー
背景:
- 血管内皮は,イシュケミア/再注射および炎症細胞による酸化ストレスに直面します.
- 水素過酸化物が,内皮細胞のカルシウム (Ca2+) 信号伝達にどのように影響するか理解するために,研究が必要である.
研究 の 目的:
- 内皮のCa2+シグナル伝達に対する過酸化水素の影響を調査する.
- 細胞内Ca2+レベルと振動に対する過酸化水素の濃度に依存する効果を決定する.
主な方法:
- 人体大動脈内皮細胞にインド1を注入し,異なる濃度の過酸化水素 (1マイクロモル/Lから10ミモル/L) に曝露した.
- 細胞内Ca2+濃度は,光器を用いて測定した.
- 細胞内Ca2+貯蔵庫は,カフェイン,ヒスタミン,またはタプシガージンを使用して,Ca2+放出源を評価するために枯渇させられました.
主要な成果:
- 低濃度の過酸化水素 (1~10マイクロモル/L) は,細胞内Ca2+に効果がなかった.
- 100ミクロモール/Lの過酸化水素は,Ca2+の漸進的な増加を誘導し,その後に繰り返されるCa2+振動と持続的な平原が続いた.
- Ca2+の振動は,エンドプラズマ網膜のCa2+貯蔵庫の放出から発生し,貯蔵庫が枯渇した後に廃絶されることが証明されています.
結論:
- 過酸化水素は,ヒト内皮細胞の濃度に依存する細胞内Ca2+振動を誘発する.
- これらの振動は,エンドプラズマ網膜の貯蔵物からCa2+が放出されることによるものです.
- 水素過酸化物のマイクロモラー濃度は,後発血性内皮機能不全に寄与する可能性があります.
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