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関連する概念動画

Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Elongation Factors02:35

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
Transcription Elongation Factors02:35

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...

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関連する実験動画

Updated: May 12, 2026

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
09:21

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

FACTは,核個体を通してトランスクリプトの延長を促進する因子です.

G Orphanides1, G LeRoy, C H Chang

  • 1Howard Hughes Medical Institute, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854, USA.

Cell
|March 7, 1998
PubMed
まとめ
この要約は機械生成です。

染色体改造は転写の開始を可能にしますが,生産的な転写延長のために,FACTタンパク質が核細胞誘発のブロックを克服する必要があります. FACTはRNAポリメラーゼIIが核細胞を通過することを促進する.

さらに関連する動画

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
07:05

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Published on: September 8, 2021

関連する実験動画

Last Updated: May 12, 2026

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
09:21

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
07:05

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Published on: September 8, 2021

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝子発現の表現について
  • クロマチンの構造 クロマチンの構造

背景:

  • RNAポリメラーゼIIによる転写は,クロマチンによって調節される基本的なプロセスです.
  • クロマチンの改造は,転写の開始に不可欠ですが,延長を妨げることができます.

研究 の 目的:

  • クロマチンのテンプレートから生産的な転写に必要な要因を調査する.
  • 転写ブロックの克服に伴う補助的要因を特定し,特徴づけること.

主な方法:

  • クロマチンテンプレートのインビトロ組立.
  • ヒトの一般転写因子とRNAポリメラーゼIIによる転写の再構成.
  • 新型付属因子の浄化と生化学的特徴付け.

主要な成果:

  • アクティベータ誘発のクロマチンの改造は,プレイニシエーション複合体の形成とイニシエーションを促した.
  • RNAポリメラーゼIIは,改造されたクロマチンの開始後に生産的な転写へのブロックに遭遇しました.
  • 浄化された因子であるFACT (クロマチンの転写を促進する) は,このブロックを克服するために不可欠であると特定されました.
  • FACTは,RNAポリメラーゼIIが核細胞を通過することを促進し,トランスクリプトの延長を可能にします.

結論:

  • クロマチン経由の生産的な転写には,単なる開始以上のものが必要です.
  • FACTは,新種のタンパク質因子であり,トランスクリプト延長に対する核細胞誘発のブロックを克服するために不可欠である.
  • FACTは,RNAポリメラーゼIIの核細胞DNAによるプロセシビティを促進する上で重要な役割を果たします.