FACTは,核個体を通してトランスクリプトの延長を促進する因子です
G Orphanides1, G LeRoy, C H Chang
1Howard Hughes Medical Institute, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854, USA.
Cell
|March 7, 1998
まとめ
染色体改造は転写の開始を可能にしますが,生産的な転写延長のために,FACTタンパク質が核細胞誘発のブロックを克服する必要があります. FACTはRNAポリメラーゼIIが核細胞を通過することを促進する.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子発現の表現について
- クロマチンの構造 クロマチンの構造
背景:
- RNAポリメラーゼIIによる転写は,クロマチンによって調節される基本的なプロセスです.
- クロマチンの改造は,転写の開始に不可欠ですが,延長を妨げることができます.
研究 の 目的:
- クロマチンのテンプレートから生産的な転写に必要な要因を調査する.
- 転写ブロックの克服に伴う補助的要因を特定し,特徴づけること.
主な方法:
- クロマチンテンプレートのインビトロ組立.
- ヒトの一般転写因子とRNAポリメラーゼIIによる転写の再構成.
- 新型付属因子の浄化と生化学的特徴付け.
主要な成果:
- アクティベータ誘発のクロマチンの改造は,プレイニシエーション複合体の形成とイニシエーションを促した.
- RNAポリメラーゼIIは,改造されたクロマチンの開始後に生産的な転写へのブロックに遭遇しました.
- 浄化された因子であるFACT (クロマチンの転写を促進する) は,このブロックを克服するために不可欠であると特定されました.
- FACTは,RNAポリメラーゼIIが核細胞を通過することを促進し,トランスクリプトの延長を可能にします.
結論:
- クロマチン経由の生産的な転写には,単なる開始以上のものが必要です.
- FACTは,新種のタンパク質因子であり,トランスクリプト延長に対する核細胞誘発のブロックを克服するために不可欠である.
- FACTは,RNAポリメラーゼIIの核細胞DNAによるプロセシビティを促進する上で重要な役割を果たします.
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