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関連する概念動画

The Nucleosome02:33

The Nucleosome

15.0K
DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
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The Nucleosome Core Particle02:10

The Nucleosome Core Particle

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Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
12.1K
Nucleosome Remodeling02:54

Nucleosome Remodeling

8.7K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
8.7K
The Nucleosome01:19

The Nucleosome

3.9K
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
3.9K
Nucleic Acid Structure01:25

Nucleic Acid Structure

8.1K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
8.1K
The Nucleosome Core Particle01:12

The Nucleosome Core Particle

2.6K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
2.6K

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関連する実験動画

Updated: Apr 28, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA
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Analyzing and Building Nucleic Acid Structures with 3DNA

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核のNFATC1/DNA複合体の溶液構造について

P Zhou1, L J Sun, V Dötsch

  • 1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

Cell
|March 20, 1998
PubMed
まとめ
この要約は機械生成です。

活性化されたT細胞 (NFAT) のDNA結合ドメインの核因子は,DNAに結合すると構造的変化を起こし,遺伝子調節を促進します. この構造的な柔軟性は,タンパク質の相互作用と遺伝子転写の鍵です.

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A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
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A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

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Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
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Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

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A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
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科学分野:

  • 分子生物学は分子生物学である.
  • 構造生物学 構造生物学とは
  • 免疫学 免疫学とは

背景:

  • 活性化されたT細胞の核因子 (NFAT) 転写因子は,サイトカインの遺伝子発現を調節する.
  • NFATタンパク質は,抗原反応性遺伝子のプロモーター/エンハンサー領域に結合し,しばしば他のDNA結合パートナーと結合する.

研究 の 目的:

  • 人間のNFATC1DNA結合ドメインとARRE2DNAサイト間のバイナリ複合体の溶液構造を決定する.
  • DNA結合がNFAT構造と転写調節におけるその役割にどのように影響するかを理解する.

主な方法:

  • NFATC1 DNA結合ドメイン/ARRE2 DNA複合体の溶液構造の決定.
  • DNA結合によって誘発される構造変化の分析.

主要な成果:

  • バイナリNFATC1-DNA複合体の構造が解明されました.
  • DNA結合は,NFATCの重要な構造要素の折り畳みを誘導する1.
  • これらの要素は,配列特異のDNA認識とタンパク質-タンパク質の相互作用に不可欠です.
  • バイナリ複合体のドメイン方向は,三元複合体のドメイン方向と異なるので,形状の変化が示唆される.

結論:

  • DNA結合は,NFATのDNA結合領域で重要な構造的再編成を誘導する.
  • これらの再編成は,遺伝子調節におけるNFAT機能に不可欠である.
  • NFATは,協力的な転写複合体を形成すると,DNA結合ドメインの方向性を変更することがあります.