ドロソフィラの免疫反応におけるRelタンパク質の規制された核輸入

L P Wu¹, K V Anderson

¹Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.

Nature

|March 24, 1998

まとめ

ドロソフィラの免疫反応には,Relタンパク質 Dif と Dorsal が関与しています. 研究者らは,DifのためにToll経路は不要であることを発見しました.

科学分野:

* 分子生物学 * 分子生物学
* 免疫学について
* 遺伝学について

背景:

*果物ハエ,ドロソフィラは,Rel/NF-kappaB転写因子を含む哺乳類と先天的な免疫応答経路を共有しています.
*2つの主要なRelタンパク質であるDifとDorsalは,感染中に脂肪細胞の核に移動します.
* トール・パスウェイはドーサルの核輸入を規制しているが,ディフに対する役割は不明である.

研究の目的:

* ドロソフィラの免疫反応中の Dif の核輸入における Toll 経路の役割を調査する.
*Relタンパク質の調節と抗菌ペプチド誘導に関与する新しい遺伝子とシグナル伝達成分を特定する.

主な方法:

*免疫反応経路に影響を与えるドロソフィラ変異体の遺伝子分析.
*感染に対する反応としてRelタンパク質 (DifとDorsal) の局所化の検査.
* サイトプラズマの保持と分解におけるカクタスタンパク質の役割の調査.

主要な成果:

* Dif.の核輸入には,通行料の経路は必要ありません.
*DorsalとDifの両方の核輸入は,カクタスのタンパク質の分解を含んでおり,異なるシグナル伝達経路を示唆しています.
*抗菌ペプチド遺伝子誘導とRelシグナル伝達に不可欠な6つの新しい遺伝子の特定.

結論:

* 明確なシグナル伝達経路は,カクタスをターゲットにすることで,DifとDorsalの核輸入を調節する.
* Rel媒介の免疫信号伝達経路の新しい成分が特定されました.
* 新しいメカニズムは,構成的な核局所化変異によって示されるように,Relタンパク質の活性を調節する可能性があります.

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