病原体に反応するトランスクリプションおよびトランスクリプション後の植物遺伝子サイレンシング

Al-Kaff¹, Covey, Kreike

¹John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK.

Science (New York, N.Y.)

|April 8, 1998

まとめ

油菜ラップのような植物は,ウイルスの遺伝子発現を変えることで,ウイルス感染症と闘うことができます. 転写および転写後の変化を含むこの防御反応は,全身感染を制御し,症状を管理するのに役立ちます.

科学分野:

植物病理学植物病理学
分子生物学は分子生物学である.
ウイルス学ウイルス学ウイルス学

背景:

植物には病原菌の侵入に対する防御メカニズムがあります.
系統的感染症は,植物の反応によって制御することができます.
カリフラワーモザイクウイルス (CaMV) は,植物に影響を与えるDNAウイルスです.

研究の目的:

系統的なカリフラワーモザイクウイルス感染に対するBrassica napus (油菜ラップ) の反応を調査する.
感染中にウイルスの遺伝子発現の調節を理解する.
ウイルスの配列ホモロジーによるトランスゲンへの影響を調べる.

主な方法:

ブラシカ・ナプス (Brassica napus) の花菜モザイクウイルスによる全身感染を誘発する.
ウイルスの遺伝子発現と症状の発達のモニタリング.
転写および転写後の規制メカニズムを分析する.

主要な成果:

ウイルスの遺伝子発現は最初は強化され,その後抑制された.
症状の発現は,ウイルスの遺伝子発現に並行して変化した.
ウイルスの配列に同質性を持つトランスゲンも,植物の反応によって影響を受けた.

結論:

ブラシカ・ナプス (Brassica napus) は,カリフラワーのモザイクウイルス感染に対するダイナミックな反応を示しています.
トランスクリプションとポストトランスクリプションの両方のメカニズムは,この応答を媒介する.
この現象には,高度に発現する遺伝子の要素の調節が含まれている可能性があります.

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