ニューロピリン-1は,血管内皮成長因子のためのアイソフォーム特異的受容体として,内皮細胞および腫瘍細胞によって発現します
S Soker1, S Takashima, H Q Miao
1Department of Surgery, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Cell
|April 7, 1998
まとめ
ニューロピリン-1は,第3の血管内皮成長因子 (VEGF) 受容体として作用し,特にVEGF165.5を結合します. この相互作用は,VEGF165がKDRと結合することを強化し,血管新生と内皮細胞の活動を調節します.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- 血管内皮成長因子 (VEGF) は血管新生に不可欠であり,主にKDR/Flk-1およびFlt-1受容体を通して信号を送信します.
- 腫瘍細胞は様々な成長因子受容体を発現し,腫瘍の成長と転移に影響を与えます.
研究 の 目的:
- VEGF.のための新しい受容体を特定し,特徴づけること.
- 血管新生における新たに特定されたVEGF受容体の役割を調査する.
主な方法:
- 腫瘍細胞から新しいVEGF受容体の浄化と発現クローニング.
- 結合と機能的活動を評価するために,KDRとの共表現研究.
- VEGFシグナル伝達におけるニューロピリン-1の役割を決定するための抑制アッセイ.
主要な成果:
- 3番目のVEGF受容体はVEGF165を結合するがVEGF121を結合しない.
- この受容体はヒトのニューロピリン-1. 1と同一であることが判明しました.
- ニューロピリン-1は,KDRへのVEGF165結合とVEGF165媒介の化学作用を強化した.
- ニューロピリン-1結合を阻害することで,内皮細胞に対するVEGF165のミトジェニック活性が低下した.
結論:
- ニューロピリン-1は,新しいVEGF受容体として機能し,VEGF165をKDR.に結合させ,VEGF165を調節する.
- ニューロピリン-1は,VEGF誘発の血管新生と内皮細胞機能の調節に重要な役割を果たします.
関連する概念動画
Paracrine Signaling
Paracrine signaling allows cells to communicate with their immediate neighbors via secretion of signaling molecules. Such a signal can only trigger a response in nearby target cells because the signal molecules degrade quickly or are inactivated if not taken up. Prominent examples of paracrine signaling include nitric oxide signaling in blood vessels, synaptic signaling of neurons, the blood clotting system, tissue repair/wound healing, and local allergic skin reactions. Nitric oxide as a...
Mechanism of Angiogenesis
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
Regulation of Angiogenesis and Blood Supply
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Introduction to Fibroblasts
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
Fibronectins Connect Cells with ECM
Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
Renewal of Intestinal Stem Cells
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...


