酵母におけるメッセンジャーRNAの成熟への転写のカップリング終結
C E Birse1, L Minvielle-Sebastia, B A Lee
1Sir William Dunn School of Pathology, South Parks Road, University of Oxford, Oxford OX1 3RE, UK.
Science (New York, N.Y.)
|May 2, 1998
まとめ
メッセンジャーRNA (mRNA) の3'-end処理と転写終結は直接関連しています. ポリアデニル化因子ではなく,割れ分因子は,CYC1遺伝子のこれらのプロセスの結合に不可欠です.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- メッセンジャーRNA (mRNA) 処理は遺伝子発現に不可欠である.
- トランスクリプションの終結は,遺伝子調節における重要なステップです.
- mRNA3端処理と転写終結の相互作用は完全に理解されていません.
研究 の 目的:
- mRNA3端処理と転写終了の間の直接的な関連性を調査する.
- この関連性における特定の処理因子の役割を決定する.
主な方法:
- Saccharomyces cerevisiaeのCYC1遺伝子を利用しました.
- mRNAの分裂とポリアデニレーションの要因をコードする遺伝子の変異を導入した.
- これらの変異が転写終結に与える影響を評価した.
主要な成果:
- 分割因子 (RNA14,RNA15,PCF11) の変異により,転写終了が妨げられました.
- ポリアデニレーション因子 (PAP1,FIP1,YTH1) の変異は,終結に最小限の影響を及ぼした.
- 分割因子と転写終結の間の直接的なリンクを確立しました.
結論:
- クリーバージ・ファクターは,トランスクリプション・ターミネーションとプレ-mRNA 3 -end 処理の結合において重要な役割を果たします.
- この連結は,初期裂解のステップに関与する要因によって媒介されます.
- RNAポリメラーゼIIの転写終結は,mRNA3端の分裂機構によって直接影響を受けます.
さらに関連する動画
関連する概念動画
01:31Translation
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
00:43Bacterial RNA Polymerase
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
01:44Termination of Translation
The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
01:53Bacterial Transcription
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
01:31Translation
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
01:44Termination of Translation
The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...