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関連する概念動画

Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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関連する実験動画

Updated: May 12, 2026

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Published on: September 24, 2015

免疫電子顕微鏡によるクロマチン構造の可視化

M Bustin, D Goldblatt, R Sperling

    Cell
    |February 1, 1976
    PubMed
    まとめ
    この要約は機械生成です。

    免疫電子顕微鏡では,クロマチンの色素が検出されました.

    さらに関連する動画

    Immunofluorescent Staining for Visualization of Heterochromatin Associated Proteins in Drosophila Salivary Glands
    10:13

    Immunofluorescent Staining for Visualization of Heterochromatin Associated Proteins in Drosophila Salivary Glands

    Published on: August 21, 2021

    Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
    14:56

    Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

    Published on: May 20, 2022

    関連する実験動画

    Last Updated: May 12, 2026

    Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
    13:06

    Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

    Published on: September 24, 2015

    Immunofluorescent Staining for Visualization of Heterochromatin Associated Proteins in Drosophila Salivary Glands
    10:13

    Immunofluorescent Staining for Visualization of Heterochromatin Associated Proteins in Drosophila Salivary Glands

    Published on: August 21, 2021

    Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
    14:56

    Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

    Published on: May 20, 2022

    科学分野:

    • 分子生物学は分子生物学である.
    • 細胞生物学 細胞生物学
    • バイオケミストリー バイオケミストリー

    背景:

    • クロマチンの構造は,DNAの包装と遺伝子調節を理解するのに不可欠です.
    • 以前のモデルでは,クロマチンの構成要素の様々な配置が提案されていたが,直接的な視覚化は限られていた.

    研究 の 目的:

    • 免疫電子顕微鏡を用いてクロマチンの構造的組織を解明する.
    • クロマチン粒子内のヒストンおよび非ヒストンタンパク質の分布と役割を調査する.

    主な方法:

    • 免疫電子顕微鏡を用いて,クロマチンとヒストンH2Bに対する抗体を用いた.
    • クロマチンのサンプルを直接視覚化し,フェリチンにラベルを貼った二次抗体を用いて抗体結合を評価した.

    主要な成果:

    • クロマチンは,直径約104 Åの密集した球形粒子 (ビーズ) として現れます.
    • 抗体結合により粒子の直径が ~300 Å に大幅に増加し,抗体包囲のモデルと一致しました.
    • 珠の95%以上は,抗クロマチン血清 (非ヒストンタンパク質を標的とする) と反応し, ~90%は,抗ヒストンH2B抗体と反応した.

    結論:

    • クロマチンは,ヒストンH2Bと様々な非ヒストンタンパク質を含んでいる可能性が高い,離散的なビーズ状の構造に編成されています.
    • この発見は,クロマチンの数珠がクロマチンの組織の基本的単位であるモデルを支持する.
    • 非ヒストンタンパク質は,クロマチンのビーズに異質に分布しているようです.