ダイネインの腕は振動力発電機を振動させるものです
C Shingyoji1, H Higuchi, M Yoshimura
1Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Japan. chikako@biol.s.u-tokyo.ac.jp
Nature
|June 26, 1998
まとめ
フラゲラ運動を推進するモータータンパク質ダイネインは,固有の振動力生成を示す. 数少ないダイネインアームでも観察されるこの振動は,真核生物のフラゲラビートに根本的な役割を果たす可能性がある.
科学分野:
- バイオフィジックス 生物物理学
- 細胞生物学 細胞生物学
- 分子モーターは分子モーターです.
背景:
- ユカリオットフラゲラは,細胞機能に不可欠なリズム運動を生成します.
- ダイネイン運動タンパク質は,フラジェラ鼓動を動かす責任があります.
- ダイネインの固有の性質とアクソネームの振動における役割は完全に理解されていません.
研究 の 目的:
- 振動がダイネインの固有の性質であるかどうかを調査する.
- ダイネイン駆動の振動に無傷なアクソネームが必要かどうかを判断する.
- ダイネインアームの個人または小集団の力発生と振動的行動を特徴付ける.
主な方法:
- 光学トラッピングナノメーターを用いて,ダイネインアームによって生成される力を測定した.
- 隔離されたダブルレットマイクロチューブルは,ダイネイン運動活動のためのトラックとして使用されました.
- 檻に閉じ込められたATPの光分解を用いた活性化ダイネインアームで,力発生測定を開始する.
主要な成果:
- 数個のダイネインアームは ~6pNのピーク力を発生させ,微小管を過程的に動かす.
- 観測された振動力と位移は,ピークからピークまでの力が約2pNで,振幅は約30nmです.
- 振動周波数 (~0.75 mM ATPで70 Hz) はATP濃度に依存し,内部のダイネインアームだけで観察された.
結論:
- ダイネインの腕は,完全なアクソネーム構造から潜在的に独立している内在の振動力生成を示します.
- データは,ダイネインの腕の振動が,フラジェラ鼓動の根本的なメカニズムであることを示唆しています.
- この発見は,生物学的動きを駆動する分子モーターの基本的な力学についての洞察を提供します.
さらに関連する動画
関連する概念動画
Static and Kinetic Frictional Force
One of the simpler characteristics of sliding friction is that it is parallel to the contact surfaces between systems, and is always in a direction that opposes the motion or attempted motion of the systems relative to each other. If two systems are in contact and moving relative to one another, then the friction between them is called kinetic friction. For example, kinetic friction slows a hockey puck sliding on ice.
However, if two systems are in contact and are stationary relative to one...
However, if two systems are in contact and are stationary relative to one...
Mechanism of Ciliary Motion
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
Mechanism of Ciliary Motion
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
Excitation-Contraction Coupling in Skeletal Muscles
Excitation-contraction coupling is a series of events that occur between generating an action potential and initiating a muscle contraction. It occurs at the triad, a structure found in skeletal muscle fibers that comprise a T-tubule and terminal cisternae of the sarcoplasmic reticulum on each side. These triads are visible in longitudinally sectioned muscle fibers. They are typically located at the A-I junction — the junction between the A and I bands of the sarcomere.
When an action potential...
When an action potential...
Muscles that Move the Arm
Nine muscles are involved in arm movements. Two of these, the pectoralis major and latissimus dorsi, originate from the axial skeleton and are called axial muscles. The other seven originate from the scapula and are called the scapular muscles.
The pectoralis major has two origins. Its clavicular head originates on the medial half of the clavicle. In contrast, the sternocostal head originates on the costal cartilages of ribs 1-6, the sternum, and the aponeurosis of the external oblique of the...
The pectoralis major has two origins. Its clavicular head originates on the medial half of the clavicle. In contrast, the sternocostal head originates on the costal cartilages of ribs 1-6, the sternum, and the aponeurosis of the external oblique of the...
Muscles that Move the Forearm
The muscles that move the forearms can be divided into four groups: forearm flexors, forearm extensors, forearm pronators, and forearm supinators. The flexors and extensors act on the elbow joint, while the pronators and supinators act on the radioulnar joints.
Forearm Flexors
The biceps brachii, brachialis, and brachioradialis are forearm flexors. The biceps brachii is made up of two heads. Its long head originates at the supraglenoid tubercle of the scapula, whereas that of the short head is...
Forearm Flexors
The biceps brachii, brachialis, and brachioradialis are forearm flexors. The biceps brachii is made up of two heads. Its long head originates at the supraglenoid tubercle of the scapula, whereas that of the short head is...


