ドロソフィラの翼における成長と細胞分裂の調整

T P Neufeld¹, A F de la Cruz, L A Johnston

¹Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.

Cell

|July 10, 1998

まとめ

ドロソフィラの翼の細胞分裂速度の変化は,組織成長に影響を与えませんでした. 代わりに,細胞分裂の変化は,細胞サイズの変化によって補償され,細胞サイクル制御は成長とは分離されていることを示しています.

科学分野:

発達生物学発達生物学とは
細胞循環の調節細胞循環の調節細胞循環の調節
ドロソフィラ・メラノガスターの研究

背景:

細胞分裂と成長は,通常,ほとんどの組織で協調されます.
細胞サイクル制御と組織成長を結びつけるメカニズムを理解することは極めて重要です.

研究の目的:

ドロソフィラの翼における細胞分裂率と組織成長の関係を調査する.
細胞増殖の操作が全体的な組織サイズに影響するかどうかを判断する.

主な方法:

遺伝子操作を用いたドロソフィラの翼クローンとコンパートメントにおける細胞分裂率の変化.
細胞数,細胞サイズ,全体的な組織成長に対する変化した増殖の影響を測定した.

主要な成果:

dE2Fの過剰生産は,サイクリンEとStringの発現を増やすことで細胞増殖を加速した.
dE2Fの減少またはRBFのレベルの増加は,細胞増殖を遅らせます.
細胞分裂速度の変化 (4〜5倍) は,組織サイズに最小限の影響を及ぼしたが,細胞サイズを著しく変化させた.

結論:

dE2FとRBFは主に細胞サイクル制御を調節するが,組織の成長を調節しない.
加速された細胞サイクルだけでは,組織成長を刺激するには不十分です.
dE2Fの活動は,成長と細胞分裂を調整するために調節することができます.

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