PCAFヒストンアセチラゼ複合体内のヒストンのようなTAFは,PCAFヒストンアセチラゼ複合体内のヒストンのようなTAFです
V V Ogryzko1, T Kotani, X Zhang
1Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
Cell
|July 23, 1998
まとめ
転写と細胞サイクル調節に関与するPCAF複合体は,TFIIDで見つかったTBP関連因子 (TAF) を含む20以上のタンパク質を含んでいます. これは,これらの転写調節複合体間の共通の機能的役割を示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- エピジェネティクス エピジェネティクス
- 遺伝子規制 遺伝子規制
背景:
- PCAFヒストンアセチラゼは,転写,細胞サイクル進行,分化を調節するために重要である.
- PCAFの分子機構を理解することは,PCAFの規制作用を解読するために不可欠です.
研究 の 目的:
- PCAF複合体のタンパク質組成を調べるために.
- TFIIDのような既知の転写因子との新しいサブユニットとその関係性を特定する.
主な方法:
- PCAF複合体の生化学分析. PCAF複合体の生化学分析. PCAF複合体の生化学分析. PCAF複合体の生化学分析.
- 質量スペクトロメトリー (暗示) のような技術を使用して,タンパク質サブユニットの識別.
- サブユニット相互作用の特徴.
主要な成果:
- PCAF複合体は20以上の異なるポリペプチドで構成されています.
- いくつかのサブユニットは,TFIID複合体の構成要素であるTBP関連因子 (TAF) と同一である.
- PCAF複合体は,ヒストンH3およびH2B型のサブユニット (hTAF (II) 31およびhTAF (II) 20/15) を含むヒストン折れを含む因子を含み,TFIIDと共有されています.
- 新しいサブユニットが特定されました:ヒストンH4のようなサブユニットがhTAFと相互作用します (II) 31とhTAFに似たWD40のリピートを含むサブユニット (II) 100.
結論:
- PCAF複合体は,TFIIDと構造的に,そして潜在的に機能的に大きな類似性を共有しています.
- TAFsとヒストンの折りたたみを含む因子の存在は,基礎転写の開始におけるPCAFの役割を示唆しています.
- 新規のサブユニットは,PCAF複合体の既知のアセチラゼ活性を超えたユニークな調節機能を示しています.
関連する概念動画
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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
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The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
01:12The Nucleosome Core Particle
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Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...