double-timeは,PERIODのタンパク質蓄積を調節する新しいドロソフィラクロック遺伝子です
J L Price1, J Blau, A Rothenfluh
1Laboratory of Genetics and National Science Foundation Science and Technology Center for Biological Timing, The Rockefeller University, New York, New York 10021, USA.
Cell
|July 23, 1998
まとめ
新しいドロソフィラ遺伝子,ダブルタイム (dbt) を特定し,昼夜リズムに決定的な役割を果たしました. ミュータントは日々のサイクルを乱し,重要なクロックタンパク質に影響を与え,dbtを強調します.
科学分野:
- *クロノバイオロジーと分子遺伝学.
- * ドロソフィラ・メラノガスターの昼夜リズムに関する遺伝的基礎の研究.
背景:
- *日経リズムは,相互作用するタンパク質を含む複雑な分子時計によって調節されます.
- * 期間 (per) と無時間 (tim) の遺伝子は,ドロソフィラの昼夜時計の重要な構成要素である.
研究 の 目的:
- *新型のドロソフィラ遺伝子,ダブルタイム (dbt) を特徴付けるため,日中時計機能に関与している.
- * PERおよびTIMタンパク質の安定性および蓄積を調節するDOUBLETIMEタンパク質の役割を明らかにする.
主な方法:
- *3つのdbtアレル (dbtS,dbtL,dbtP) の分離と特徴付けについて.
- *変異性ドロソフィラの行動リズムと分子振動の分析.
- * 幼虫におけるPERとTIMタンパク質の蓄積とリン酸化状態の評価.
主要な成果:
- * dbtSとdbtLの変異種は,行動と分子昼夜リズムが変化している.
- * dbtPアレルは,子犬の死亡率を引き起こし,PERとTIMの昼夜サイクルを廃止する.
- * dbtP変異体では,PERタンパク質が構成的に蓄積し,TIMとは独立して低酸化される.
- dbtP変異体は,TIMと独立して構成的なPER蓄積と低酸化を示しています.
結論:
- * DOUBLETIMEタンパク質は通常,PERタンパク質の安定性と蓄積を低下させる.
- * この機能は,PER/timトランスクリプションとPER/TIM複合体の活動との間の時間遅延に不可欠であり,分子リズム性を確保します.
- * dbtは,ドロソフィラの昼間時計機構の重要な調節剤である.
関連する概念動画
00:53Transgenic Organisms
Overview
02:05Gene Evolution - Fast or Slow?
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
02:50Dosage Compensation
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will have...
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will have...
00:53Transgenic Organisms
Overview
02:05Gene Evolution - Fast or Slow?
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
01:20Genetic Material
Within the human body, a complex and detailed system of trillions of cells works in unison to sustain life. Each cell houses a nucleus, which contains 46 chromosomes divided into 23 pairs. Chromosomes are highly coiled structures made of the genetic material DNA. These chromosomes are essential carriers of genetic information, with half inherited from the mother through her egg and the other half from the father's sperm, combining to create the unique genetic makeup of an individual.