DishevelledはJNKを活性化し,平面的極性におけるJNK経路と無翼のシグナリングを区別する
M Boutros1, N Paricio, D I Strutt
1European Molecular Biology Laboratory, Developmental Biology Programme, Heidelberg, Germany.
Cell
|July 23, 1998
まとめ
変形したタンパク質はJNK信号伝達経路を活性化します. 異なるWnt/Frizzled信号は,異なるDishevelledドメインを使用して,平面の極性および形態変異を調節します.
科学分野:
- 発達生物学 発達生物学とは
- 細胞シグナリング
- 分子遺伝学 分子遺伝学
背景:
- フリズリングタンパク質は,Wntシグナル分子の受容体として機能する.
- ドロソフィラのFrizzledタンパク質は,平面的極性や形態変異のような独特の発達プロセスに関与しています.
- Dishevelledは,Wnt/Frizzledの両方のシグナル伝達経路に必要な重要なタンパク質です.
研究 の 目的:
- Wnt/Frizzledのシグナル伝達経路におけるDishevelledの役割を調査する.
- DishevelledがJNKシグナル伝達のような細胞内カスケードをどのように活性化するかを判断するために.
- 異なるWnt/Frizzled媒介プロセスにおけるDishevelledの特定のドメイン要件を解明する.
主な方法:
- ドロソフィラの遺伝子解析.
- 過剰表現に関する研究.
- 発達障害の救済分析. 発達障害の救済分析.
- アレル特異変異分析. アレル特異変異分析.
主要な成果:
- DishevelledはJNKシグナルキャスケードを活性化することが示されました.
- Dishevelledの明確なタンパク質ドメインは,平面的極性および形態変異におけるその機能のために必要です.
- DishevelledのC端末DEPドメインは,平面極性欠陥を救済し,JNKシグナリングを誘発するために不可欠です.
- 平面的極性に影響を与える特定のアレル,dsh1は,DEP領域で変異していることが判明しました.
結論:
- 異なるWnt/Frizzled信号は,異なる細胞内経路を活性化する.
- Dishevelledは信号トランスデューサーとして機能し,特定のドメインの相互作用を通じてWnt/Frizzled信号を区別します.
- DishevelledのDEPドメインは,平面極性およびJNK活性化のためのWnt/Frizzled信号伝導を媒介する上で重要な役割を果たしています.
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