lefty-1は,lefty-2とノダルのレギュレータとして,左-右の決定に必要です
1Division of Molecular Biology, Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.
Cell
|August 26, 1998
まとめ
lefty-1が欠けていた突然変異のマウスは,内臓器の欠陥と胸の左アイソメリズムを示した. これは, lefty-1がノダル表現を左側に制限し, lefty-2が左右の決定に不可欠であることを示唆しています.
科学分野:
- 発達生物学 発達生物学について
- 遺伝学 遺伝学とは
- 胚学 胚学について
背景:
- 左-右 (L-R) 決定は,胚の発達における基本的なプロセスである.
- lefty-1, lefty-2, nodalなどの遺伝子は,L-R非対称性の確立に関与することが知られている.
- Pitx2は,通常左側で発現する,左側性にとって重要なホメオボックス遺伝子です.
研究 の 目的:
- 左から右の決定における lefty-1 の特定の役割を調査する.
- 発育中のマウスの胚における lefty-1 欠乏の影響を分析する.
主な方法:
- lefty-1遺伝子 (lefty-1-/-) を欠いている突然変異のマウスの生成と分析.
- 内臓器官の位置の欠陥の観察と文書化.
- 遺伝子発現パターン (ノダル,レフティ2,ピトックス2を含む) を変異胚で調べる.
主要な成果:
- 左-1欠乏マウスの内臓器に左-右の位置欠陥が観察されました.
- 胸の左側アイソメリスは,左側-1-/-マウスの最も頻繁なフェノタイプでした.
- lefty-1の欠如は,ノダル,lefty-2,およびPitx2の双方向発現につながり,正常な左側非対称性を破壊しました.
結論:
- Lefty-1は, lefty-2の発現を制限する上で重要な役割を果たし,胚の発達の左側にノダルの発現を制限する.
- Lefty-2またはノダルは,左側性を決定する重要な信号を暗号化している可能性があります.
- lefty-1機能の障害により,異常なL-Rパターンが形成され,胚の発達におけるL-Rの重要性を強調する.
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