LIN-10は,神経細胞と上皮質における偏光タンパク質の局所化経路の共通の構成要素である
C Rongo1, C W Whitfield, A Rodal
1Department of Molecular and Cell Biology, University of California, Berkeley 94720-3200, USA.
Cell
|September 30, 1998
まとめ
神経細胞と上皮細胞は,タンパク質分類経路を共有しています. PDZタンパク質LIN-10は,両方の細胞タイプにおけるグルタミン酸受容体 (GLR-1) の局所化に不可欠であり,共通のメカニズムを示しています.
科学分野:
- 細胞生物学 細胞生物学
- 神経科学は神経科学である.
- 分子生物学は分子生物学である.
背景:
- 分極化されたタンパク質分類は,ニューロンと上皮細胞の両方の細胞機能に不可欠です.
- これらの異なる細胞タイプにおけるタンパク質の局所化を制御するメカニズムは完全に理解されていません.
- グルタミン酸受容体GLR-1は,極化分類の研究のためのモデルタンパク質として機能する.
研究 の 目的:
- 神経細胞と上皮細胞が,分極化されたタンパク質分類の共有メカニズムを利用しているかどうかを調査する.
- ベースラテラルおよびポストシナプスタンパク質の局所化に関与する一般的な分子成分を特定する.
- GLR-1の局所化におけるPDZタンパク質LIN-10の役割を明らかにする.
主な方法:
- C. elegans. のニューロン細胞および上皮細胞におけるGLR-1局所化の比較分析.
- GLR-1カーボキシ端末配列の遺伝子操作.
- 野生型および突然変異の背景におけるタンパク質局所化の評価.
主要な成果:
- GLR-1は,神経細胞のポストシナプス部位と,上皮細胞の基礎側膜に局限しています.
- PDZタンパク質LIN-10は,両方の細胞タイプにおけるGLR-1の局所化に必要であり,共通の経路を示唆しています.
- GLR-1のカルボキシ末端配列を改変することで,リン-10変異体におけるシナプス局所化が回復し,代替経路を示した.
結論:
- LIN-10は,ベースロラテルおよびポストシナプスタンパク質局所化経路の両方に共通する成分です.
- C. elegansニューロンは,ポストシナプスGLR-1局所化のために少なくとも2つの異なる経路を使用します.
- この研究は,異なる細胞タイプにおける偏光タンパク質の分類における保存されたメカニズムを明らかにしています.
関連する概念動画
Assembly of Complex Microtubule Structures
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
Nuclear Localization Signals and Import
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of 2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
Cell Polarization by Rho Proteins
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
Cytoskeletal Coordination in Cell Migration
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...
Anchoring Junctions
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
Catenins
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...


