SWI/SNFに関連するクロマチンの改造複合体,E-RC1は,EKLFによる組織特異の転写制御のために,in vitroで必要である
J A Armstrong1, J J Bieker, B M Emerson
1Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA.
Cell
|October 20, 1998
まとめ
エリトroid Krüppel-like factor (EKLF) は,ベータ・グロービン遺伝子を活性化するためにクロマチンの改造複合体 (E-RC1) を必要とします. このSWI/SNF関連の複合体は,遺伝子調節のためのDNA結合タンパク質と選択的に相互作用する.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子規制 遺伝子規制
- クロマチンのダイナミクス
背景:
- エリトroid Krüppel-like factor (EKLF) は,ヒトβ-グロービン遺伝子の段階特異的な発現に決定的な役割を果たしています.
- クロマチンの構造は,遺伝子のアクセシビリティと転写を調節する上で重要な役割を果たします.
研究 の 目的:
- EKLF媒介のβ-グロービン遺伝子活性化におけるクロマチンの改造の役割を調査する.
- EKLF機能に必要なコアクティベーター複合体の構成要素を特定する.
主な方法:
- クロマチンテンプレートを用いたインビトロ転写アッセイ.
- EKLFコアクティベーター再構成複合体1 (E-RC1) の生化学的特徴.
- E-RC1の転写因子およびDNA結合タンパク質との相互作用の分析.
主要な成果:
- EKLFには,SWI/SNF関連の複合体E-RC1が,クロマチンの活性β-グロービンプロモーターを生成するために必要です.
- E-RC1は,酵母 SWI/SNFサブユニット (BRG1,BAF170,BAF155,INI1) のホモログと,ユニークなサブユニットであるBAF57.1を含む.
- E-RC1は,EKLF依存の転写を活性化させる機能的選択性を示すが,TFE-3によって媒介される転写は活性化しない.
結論:
- SWI/SNFファミリーのメンバーは,転写活性化に直接参加します.
- 染色体改造複合体は,DNA結合タンパク質との選択的相互作用を通じて遺伝子サブセットを調節することができます.
- E-RC1は,β-グロービンの遺伝子発現におけるEKLFの重要な共同活性化因子である.
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