Mec1とRad53に依存するチェックポイントは,DNA複製の遅発の起源を制御する
1Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, UK.
Nature
|October 23, 1998
まとめ
ヒドロキシウレアはDNA複製フォークの進行を阻害し,芽生えた酵母菌の遅発発火を抑制します. この調節はS相内チェックポイントによって制御され,細胞生存を助長する.
科学分野:
- 分子生物学は分子生物学である.
- 細胞サイクル規制について
- DNAレプリケーション DNA複製
背景:
- ユカリオットDNAの複製は,複数の起源から始まり,S相全体で予想通り発火する.
- SフェーズにおけるDNA複製の開始と延長の間の調整は,十分に理解されていません.
研究 の 目的:
- DNA複製の開始と延長の間の調整を調査する.
- Sフェーズにおける遅発発火の規制を理解するために.
主な方法:
- 芽生える酵母をモデル生物として利用した.
- 初期の起源からの複製フォークの進行をブロックするために,適用されたヒドロキシ尿素.
- 後期発火の効果を分析した.
主要な成果:
- ハイドロキシ尿素処理は,遅い起源の発火を抑制しました.
- 遅い起源は,長い期間,イニシアチブ能力のある状態に留まっていました.
- 遅発発火の抑制は,rad53とmec1チェックポイント変異体では欠陥があった.
結論:
- 後期発火は積極的に規制され,S相内のチェックポイントとリンクされています.
- 遅発の発火の規制は,複製ストレス下での細胞生存に不可欠です.
- RAD53やMEC1のようなチェックポイント遺伝子は,遅発の発火を制御する役割を果たします.
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In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
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In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.