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クロマチンテンプレートの転写のためのRSFとFACTの要件 in vitro
G LeRoy1, G Orphanides, W S Lane
1Howard Hughes Medical Institute, Division of Nucleic Acid Enzymology, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.
Science (New York, N.Y.)
|December 4, 1998
まとめ
研究者らは,クロマチンに包装されたDNAからの遺伝子転写に不可欠な新しい因子,リモデリング・アンド・スペーシング・ファクター (RSF) を特定しました. RSFはFACTとともに,これらの複雑なテンプレート上でトランスクリプションの開始と延長を可能にします.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子規制 遺伝子規制
- クロマチンの構造 クロマチンの構造
背景:
- 裸DNAの転写開始には,RNAポリメラーゼIIと一般的な転写因子が必要です.
- クロマチンに包装されたDNAは,転写の開始に重大な障壁を提示します.
- 既存の転写因子セットは,クロマチンのテンプレートによる転写には不十分である.
研究 の 目的:
- クロマチンテンプレートでの転写開始を促進する要因を特定する.
- クロマチンのアクティベータ依存トランスクリプションの最小因子要件を定義する.
- 新種のクロマチンの転写因子の活動を特徴づける.
主な方法:
- 細胞抽出物からタンパク質因子の浄化.
- クロマチンテンプレートを用いたインビトロ転写アッセイ.
- 核粒子の改造と間隔の活動を決定するためのアッセイ.
- トランスクリプションの開始と延長における浄化された因子の機能分析.
主要な成果:
- 新しい因子であるリモデリング・アンド・スペーシング・ファクター (RSF) が精製されました.
- RSFはアデノシントライホスファートに依存した核細胞の再構築と間隔の活動を表しています.
- RSFは,クロマチンのテンプレートにおけるアクティベータ依存の転写イニシアチブを促進します.
- FACT複合体は,RSFで開始されたポリメラーゼによるトランスクリプト延長に必須です.
結論:
- この研究では,クロマチンテンプレートでの転写のための最小因数要件を定義しています.
- RSFは,染色体ベースの転写抑制を克服するための重要な要素です.
- RSFとFACTの協調的な作用は,効率的なクロマチンの転写に不可欠です.
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