受容体 Msn5 は,リン酸化転写因子 Pho4 を核の外に輸出する

A Kaffman¹, N M Rank, E M O'Neill

¹Department of Biochemistry and Biophysics, University of California at San Francisco, School of Medicine, 94143-0448, USA.

Nature

|December 16, 1998

まとめ

イーストの転写因子 Pho4 のリン酸化により,その核輸出が引き起こされます. このプロセスはMsn5受容体を利用し,タンパク質の局所化と活性を制御する重要なメカニズムを明らかにします.

科学分野:

細胞生物学細胞生物学
分子生物学は分子生物学である.
バイオケミストリーバイオケミストリー

背景:

タンパク質の核-細胞プラズマ移動は,その活動を調節する.
リン酸化は,タンパク質の局所化を制御する重要な翻訳後の修正である.
リン酸化による核輸出のメカニズムは完全に理解されていません.

研究の目的:

酵母転写因子Pho4の調節を調査する. 核輸出.
Pho4局所化の制御におけるリン酸化の役割を明らかにする.
Pho4核輸出に関与する輸出受容体を特定してください.

主な方法:

インビヴォおよびインビトロ生化学測定法.
タンパク質のリン酸化と局所化の分析.
イースト遺伝学と分子生物学技術.

主要な成果:

核からのPho4輸出は,Pho80-Pho85複合体によって媒介されるリン酸化によって誘発されます.
Msn5はインポートリンベータファミリーのメンバーで,Pho4.4の核輸出受容体として作用する.
Msn5は,Ran-GTPに依存した方法で,リン酸化したPho4と結合する.

結論:

Pho80-Pho85によるリン酸化は,Pho4の核輸出を直接制御する.
Msn5は,Pho4.4のリン酸化依存の輸出を媒介する.
この研究は,リン酸化によるタンパク質核輸出の制御のための基本的なメカニズムを明らかにしています.

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