関連する実験動画

Updated: Jun 10, 2026

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

ハイブリッド細胞におけるヒト酵素の免疫化学検出

N Shimizu, Y Shimizu, R S Kucherlapati

|January 1, 1976

まとめ

研究者らは,細胞ハイブリッドにおけるヒト酵素を検出するための抗体ベースの方法を開発した. この技術は,NADP関連イソチラート脱水素酶,フォスフォグルコースイソメラーゼ,およびHGPRTのヒト遺伝子を特定の染色体にマッピングすることに成功しました.

科学分野:

バイオケミストリーバイオケミストリー
遺伝学遺伝学とは
免疫学免疫学とは

背景:

人間特有の酵素検出は,遺伝子マッピングに不可欠です.
ソマティック細胞のハイブリデーションは,染色体に遺伝子を配分するための強力なツールです.

研究の目的:

ヒト酵素を検出するための抗体ベースの免疫降水法を開発し,検証する.
人間の細胞プラズマのNADP関連イソシトラート脱水素酶 (IDH),フォスフォグルコースイソメラーゼ (PGI),ヒポキサンチングアニンフォスフォリボシルトランスフェラーゼ (HGPRT) 遺伝子の染色体の位置を割り当てる.

主な方法:

精製されたヒトIDH,PGI,HGPRTに対するウサギの抗菌剤の生産.
人とマウスの体細胞ハイブリッドを用いた免疫プレシピテーションアッセイ.
酵素活性とヘテロジマー形成を分析するために,スターチゲル電泳.

主要な成果:

ヒト特異酵素は,ハイブリッド細胞溶解物から選択的に沈殿された.
この方法は,ヒトのホモジメとヒトとマウスのヘテロジメの両方の検出を可能にしました.
遺伝子の割り当ては,染色体2へのIDH,染色体19へのPGI,X染色体へのHGPRTが行われました.

結論:

抗体ベースの免疫降雨は,ヒト特異酵素を検出するための敏感で定量的な方法です.
この技術により,IDH,PGI,HGPRTの遺伝子がそれぞれのヒト染色体に順番に割り当てられることが可能になりました.
この発見は,以前に発表された遺伝子マッピングデータと一致しています.

さらに関連する動画

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 16, 2013

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Published on: October 20, 2018

関連する概念動画

Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.