概括
这项研究通过分析来自小鼠胚胎和髓瘤细胞的DNA片段,提供了免疫球蛋白基因体质重组的直接证据. 这些发现揭示了基因进化和细胞分化.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 免疫球蛋白基因对于适应性免疫是至关重要的.
- 了解免疫球蛋白基因组织和重排是解读免疫系统发育和多样性的关键.
研究的目的:
- 在小鼠胚胎和骨髓瘤细胞中识别和描述编码免疫球蛋白兰巴达光链的DNA片段.
- 为免疫球蛋白基因的体质重组提供直接证据.
主要方法:
- 南方凝涂抹使用合成的免疫球蛋白羊链mRNADNA转录作为探针.
- 将DNA片段克隆成一个lambda菌素载体 (lambdaWES).
- 糖凝电泳,凝涂抹和电子显微镜R循环映射用于表征.
- 异质双重分析和限制酶映射.
主要成果:
- 在胚胎DNA中识别出不同的DNA片段 (8.6,4.8,3.5 kb) 和骨髓瘤DNA中的额外片段 (7.4 kb).
- 克隆的碎片揭示了V lambdaI,C lambdaI和V lambdaII序列的特定安排.
- 骨髓瘤DNA克隆显示V lambdaI和C lambdaI被1.2kb段隔开,包括一个J序列.
- 在V-非翻译DNA边界展示了同质交换机,对应于J序列位置.
结论:
- 在克隆DNA中观察到的序列排列与细胞DNA的序列排列相似.
- 结果提供了强有力的证据,证明了免疫球蛋白基因的体质重组.
- 这些发现对理解基因进化和细胞分化有意义.
更多相关视频
12:30Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae
Published on: October 6, 2017
7.2K
08:12Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes
Published on: November 1, 2011
20.4K
相关概念视频
Recombinant DNA
103.4K
Overview
103.4K
Overview of Transposition and Recombination
19.4K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
19.4K
Gene Conversion
10.7K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
10.7K
Viral Recombination
25.2K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
25.2K
Homologous Recombination
63.3K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
63.3K
Combinatorial Gene Control
9.7K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.7K
