相关实验视频
Updated: Jul 25, 2026

16:24
Analyzing and Building Nucleic Acid Structures with 3DNA
Published on: April 26, 2013
一个与DNA结合的超比索拉克斯 - 超外形家庭主体复合体的结构
J M Passner1, H D Ryoo, L Shen
1Structural Biology Program, Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA.
Nature
|March 6, 1999
概括
同源性 (Hox) 蛋白质和异源性 (Ext) 蛋白质合作调节动物的发育. 结构分析揭示了它们与DNA以及彼此之间的相互作用如何决定前后轴形成.
科学领域:
- 发展生物学 发展生物学
- 结构生物学 结构生物学
- 遗传学 遗传学 是一个
背景情况:
- 基因表达调节对于多细胞生物的发展至关重要.
- 同源性 (霍克斯) 基因控制动物前后轴模式.
- 霍克斯蛋白需要像Extradenticle (Ext) 这样的合作伙伴来进行特定的DNA结合.
研究的目的:
- 为了阐明Hox-Extradenticle蛋白质复合体形成的结构基础.
- 要了解这个复合体如何实现特定的DNA结合和功能.
- 提供对发育路径调节的见解.
主要方法:
- 确定了一个Ultrabithorax-Extradenticle-DNA复合体的晶体结构.
- 使用最小的聚,形成一个合作的异构聚合物.
- 在2.4A分辨率下分析了蛋白质-DNA和蛋白质-蛋白质相互作用.
主要成果:
- 超比索拉克斯和超偏的同居域结合了相反的DNA面.
- 大多数合作相互作用都涉及Ultrabithorax的YPWM图案与Extradenticle结合.
- 这种相互作用定义了Hox-Extradenticle介导的动物发育的原则.
结论:
- 对于Hox-Extradenticle合作绑定来说,YPWM图案是至关重要的.
- 结构洞察力解释了通过Extradenticle增强Hox蛋白特异性的原因.
- 这种机制对于动物前后轴模式至关重要.
相关概念视频
The DNA Helix
Overview
The DNA Helix
Overview
DNA as a Genetic Template
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
Homologous Recombination
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
The DNA Helix
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
Homologous Recombination
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

