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通过NAADP协调激素诱导的Ca2+信号模式在胰腺细胞中.

J M Cancela1, G C Churchill, A Galione

  • 1Department of Pharmacology, University of Oxford, UK. j.m.cancela@liverpool.ac.uk

Nature
|March 17, 1999
PubMed
概括
此摘要是机器生成的。

尼古丁酸腺因二核酸 (NAADP) 触发胰腺细胞中的各种信号,与其他信使不同. 这表明NAADP在哺乳动物中充当了一种新的细胞内信使.

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科学领域:

  • 细胞生物学 细胞生物学
  • 生物化学 生物化学
  • 生理学 生理学 生理学

背景情况:

  • 激素和神经递质调节细胞内 (Ca2+) 的释放,创建特定的信号模式.
  • 伊诺西三酸盐 (InsP3) 和循环氨酸5'-二酸盐-核糖 (cADPR) 是已知的Ca2+信使,分别激活InsP3和氨酸受体.
  • 现有的信使并不能完全解释胰腺突细胞中胆囊托基宁 (CCK) 诱导的Ca2+信号复杂性.

研究的目的:

  • 研究尼古丁酸腺因二核酸 (NAADP) 作为胰腺细胞中潜在的Ca2+信使的作用.
  • 将NAADP的Ca2+调动效应与InsP3和cADPR进行比较.
  • 阐明CCK引起的Ca2+信号受调节的机制.

主要方法:

  • 测量胰腺状细胞中的细胞内Ca2+度.
  • 应用NAADP,cADPR和InsP3来刺激Ca2+的释放.
  • 通过使用不同度的NAADP来选择性失活CCK引起的Ca2+信号.

主要成果:

  • NAADP触发了各种Ca2+反应,包括短时间和长时间的尖峰,与InsP3和cADPR不同.
  • 与cADPR或InsP3.3相比,胰腺酸性细胞对NAADP表现出更高的敏感性.
  • 较高的NAADP度可以选择性地抑制CCK诱导的Ca2+信号传递.

结论:

  • 在胰腺细胞中,NAADP作为一种强大的Ca2+调动信使.
  • 通过NAADP介导的信号与InsP3和cADPR通路不同,并且可以调节它们.
  • NAADP代表了一种新的细胞内信使,可能参与哺乳动物细胞信号传递.