DREAM是一种Ca2+调节的转录抑制剂
A M Carrión1, W A Link, F Ledo
1Instituto de Neurobiología S. Ramón y Cajal, CSIC, Madrid, Spain.
Nature
|March 17, 1999
概括
离子 (Ca2+) 调节基因表达. 研究人员发现了DREAM,这是一种新的Ca2+结合蛋白,可以作为转录抑制剂,直接影响诸如prodynorphin和c-fos.等基因.
科学领域:
- 分子生物学分子生物学
- 神经科学是一个神经科学.
- 遗传学 是一个遗传学.
背景情况:
- 细胞内离子 (Ca2+) 流是基因表达的关键调节者.
- 虽然已知Ca2+调节的激酶和酶调节转录因子,但直接的核Ca2+效应因子仍未确定.
- 人类prodynorphin基因对记忆和疼痛至关重要,由一个沉声器序列 (DRE) 调节.
研究的目的:
- 确定参与基因表达调节的直接核Ca2+结合蛋白.
- 描述一种对Ca2+作出反应的新型转录抑制剂.
- 研究这种抑制剂在控制特定基因表达中的作用.
主要方法:
- 一种新的Ca2+结合蛋白DREAM (DRE-antagonist调节器) 的分离和表征.
- 对DREAM对下游监管元件 (DRE) 的DNA结合活性进行分析.
- 评估DREAM的转录抑制器功能,以响应Ca2+刺激及其Ca2+结合域 (EF-hands) 中的突变.
- 评估DREAM对产生诺芬和c-fos基因促进剂的影响.
主要成果:
- DREAM被确定为一种转录抑制剂,专门与DRE序列结合.
- 梦想拥有四个EF-手的Ca2+结合域.
- Ca2+刺激抑制了DREAM的DNA结合能力和抑制器功能.
- 在EF手域中的突变取消了DREAM的Ca2+依赖调节.
- 梦想抑制了来自prodynorphin和c-fos促进者的转录.
结论:
- 梦想是第一个被发现的Ca2+结合蛋白,作为直接DNA结合的转录调节器.
- DREAM调解了Ca2+依赖基因抑制,将信号与转录控制联系起来.
- 这一发现为离子如何影响基因表达提供了新的机制,特别是与神经元功能和反应相关的基因.
相关概念视频
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