在非分裂细胞中由乌拉的转录绕行引起的表型变化
A Viswanathan1, H J You, P W Doetsch
1Graduate Program in Genetics and Molecular Biology and Departments of Biochemistry and Radiation Oncology, Emory University School of Medicine, Atlanta, GA 30322, USA.
概括
在DNA中的乌拉可以导致转录过程中的突变,即使在非分裂细胞中也是如此. DNA 修复能力影响这些转录驱动突变的速率,可能产生突变蛋白质.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 细胞因子去胺化为 uracil 是一种常见的 DNA 修改.
- 在DNA中的乌拉可以在复制过程中导致G到A基的替代.
- 乌拉对体内转录忠实性的影响尚未完全理解.
研究的目的:
- 为了研究DNA中乌拉对非分裂大肠杆菌的转录忠实性的影响.
- 为了确定乌拉是否可以在体内导致突变蛋白质的产生.
- 探索DNA修复在 uracil 诱导的转录错误中的作用.
主要方法:
- 引入 uracil 进入 E. coli 中的火虫 luciferase 基因内的停止.
- 测试光酶活性以检测转录错误.
- 与突变频率相关的 uracil 修复能力的评估.
主要成果:
- 在DNA模板中Uracil的停止符号导致活跃的露西法酶,表明转录基替代.
- uracil 诱导的转录错误的程度取决于细胞的 uracil 修复能力.
- 这表明在非分裂细胞中产生突变蛋白的途径.
结论:
- DNA损伤,特别是 uracil,可以通过转录在非分裂细胞中驱动突变蛋白的产生.
- 这项研究突出了蛋白质突变发生的DNA损伤依赖的转录驱动机制.
- 尿素修复是缓解这些转录相关突变的关键因素.
相关概念视频
Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Cell Motility through Blebbing
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Forced Transdifferentiation
Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial transdifferentiation occurs...
Artificial transdifferentiation occurs...


