相关实验视频

Updated: Jul 17, 2026

Aip1p Dynamics Are Altered by the R256H Mutation in Actin
08:57

Aip1p Dynamics Are Altered by the R256H Mutation in Actin

Published on: July 30, 2014

识别和表征多种形式的行为素的识别和表征

J I Garrels, W Gibson

    Cell
    |December 1, 1976
    PubMed
    概括

    哺乳动物细胞含有多种actin形式,包括肌肉中的alphaactin和非肌肉细胞中的β/gammaactin. 这些独特的actin异型在细胞分化和功能中起着至关重要的作用.

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    Measuring Protein Binding to F-actin by Co-sedimentation

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    A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues06:54

    A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues

    Published on: June 3, 2021

    相关概念视频

    Introduction to Actin01:26

    Introduction to Actin

    Actin is a highly conserved cytoskeletal protein found abundantly in eukaryotic cells. It constitutes 10% weight of the total cellular protein in muscle cells, while in non-muscle cells, it is lower and makes up around 1–5 percent of the total cell protein. Actin found in the unicellular amoebae and complex multicellular animals is around 80% similar, demonstrating their conservation over a billion years of evolution.  Actin coding genes are conserved within species and across different species.
    Actin Polymerization01:42

    Actin Polymerization

    Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶  nucleation, elongation, and steady-state phase.
    The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight actin...
    Generation of Straight or Branched Actin Filaments01:14

    Generation of Straight or Branched Actin Filaments

    The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
    Arp2/3 Complex
    Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
    Actin Filament Depolymerization01:19

    Actin Filament Depolymerization

    Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
    In F-actin, the ADF/cofilin proteins...
    Formation of Higher-order Actin Filaments01:11

    Formation of Higher-order Actin Filaments

    The polymerization of G-actin monomers into filamentous F-actin is a multi-step process. Once the F-actins are formed, they can bundle together in different arrangements to form higher-order networks and regulate cellular functions. Common examples include the formation of lamellipodia and filopodia at the cell's leading edge by actin reorganization in a migrating cell. The microvilli on the brush border epithelial cells are also formed through the F-actin network.
    The high-order actin networks...
    Actin Polymerization and Cell Motility01:13

    Actin Polymerization and Cell Motility

    Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
    Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate.