Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Pathologic analysis of capsular and incisional denudation and positive margin status in the development of a robot-assisted laparoscopic prostatectomy program.

Journal of robotic surgery·2016
Same author

In vivo requirements for GATA-1 functional domains during primitive and definitive erythropoiesis.

The EMBO journal·2001
Same author

Hand-assisted laparoscopic radical nephrectomy: the experience of the inexperienced.

Journal of endourology·2001
Same author

Regulation of hyaluronan synthase gene expression in human periodontal ligament cells by tumour necrosis factor-alpha, interleukin-1beta and interferon-gamma.

Archives of oral biology·2001
Same author

Lessons from an international survey of paper cases of 10 real patients from an early arthritis clinic. CRI (Club Rhumatismes et Inflammation) Group.

The Journal of rheumatology·2001
Same author

Deglutitive movement of the tongue under local anesthesia.

American journal of physiology. Gastrointestinal and liver physiology·2001

相关实验视频

Updated: Jul 13, 2026

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
11:48

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Published on: July 24, 2013

改变基因顺序或位置控制区域的定向对小鼠人类β-环球蛋白基因表达的影响.

K Tanimoto1, Q Liu, J Bungert

  • 1Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208-3500, USA.

Nature
|April 7, 1999
PubMed
概括
此摘要是机器生成的。

改变人类β型环球蛋白基因的排列或它们的位置控制区域 (LCR) 会影响基因表达. 基因顺序和LCR方向对于红色素形成过程中适当的时间调节至关重要.

更多相关视频

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
08:43

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Published on: June 6, 2016

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
11:13

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

相关实验视频

Last Updated: Jul 13, 2026

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
11:48

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Published on: July 24, 2013

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
08:43

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Published on: June 6, 2016

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
11:13

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

科学领域:

  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学
  • 发育生物学 发展生物学

背景情况:

  • 人类β型环球蛋白基因 (epsilon,Ggamma,Agamma,delta,β) 被集群并由位置控制区域 (LCR) 调节.
  • 了解这些基因的空间和取决于方向的调节对于洞察红色素形成至关重要.

研究的目的:

  • 研究基因顺序和LCR定向对人类β型环球蛋白基因时间调节的功能后果.
  • 为了确定LCR活性是否依赖于其方向和接近特定的球蛋白基因.

主要方法:

  • 在转基因小鼠中利用重组技术逆转β型环球蛋白基因或LCR的顺序.
  • 在携带正常和突变转基因的小鼠中分析了全球蛋白基因的表达模式.

主要成果:

  • 在红细胞形成的黄囊阶段,epsilon-globin基因的表达需要其与LCR相邻的位置.
  • 发现LCR活动是取决于方向的,这表明它不能作为一个简单的增强剂.
  • 基因顺序或LCR方向的逆转显著改变了β型环球蛋白基因转录,而没有改变整合部位,拷贝数或转变作用因子.

结论:

  • 人类β型环球蛋白基因表达的时间调节中,LCR的空间布局和方向至关重要.
  • LCR功能比简单的增强更复杂,涉及关键的方向依赖机制,对发育基因调节至关重要.