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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

13.4K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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相关实验视频

Updated: May 5, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

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含有RD的多子单元复合体NELF与DSIF合作,抑制RNA聚合酶II延长.

Y Yamaguchi1, T Takagi, T Wada

  • 1Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.

Cell
|April 13, 1999
PubMed
概括
此摘要是机器生成的。

研究人员发现了一种新的蛋白质,负延长因子 (NELF),与DSIF一起作用,抑制RNA聚合酶II (pol II) 转录延长. P-TEFb扭转了这种压制,揭示了一个关键的监管网络.

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Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
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Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

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相关实验视频

Last Updated: May 5, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

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Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
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Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

1.7K

科学领域:

  • 分子生物学分子生物学
  • 基因规则 基因规则
  • 生物化学 生物化学

背景情况:

  • DRB是一种已知的RNA聚合酶II (pol II) 转录延长的抑制剂.
  • 两个延长因子,DSIF和P-TEFb,对于DRB在极点II的行动至关重要.
  • 了解pol II延长因子对于理解基因调节至关重要.

研究的目的:

  • 识别和描述涉及DRB敏感转录的新型蛋白质因子.
  • 阐明DRB作用的分子机制及其调节网络.
  • 为了研究一个新发现的因素在极II延长中的作用.

主要方法:

  • 从HeLa核提取物中净化蛋白质因子.
  • 生物化学测试以评估转录延长活性.
  • 蛋白质成分及其相互作用的识别.

主要成果:

  • 第三个蛋白质因子,负延长因子 (NELF) 被确定并净化.
  • NELF与DSIF合作,大力压制波II的延伸.
  • 通过pol II C终端域酸化,P-TEFb可以逆转NELF/DSIF介导的抑制.
  • NELF包括五种多,包括RD蛋白.

结论:

  • 这项研究揭示了DRB在转录延长中的作用的分子机制.
  • 一个涉及负延长因子 (NELF),DSIF和P-TEFb的监管网络控制了极II延长.
  • 这些发现为复杂的基因转录调节提供了新的见解.