普雷西尼林是Drosophila中Notch的活性和核接入所需的
1Department of Genetics and Development, Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA. struhl@cuccfa.ccc.columbia
Nature
|April 17, 1999
概括
普列尼林对于Notch信号传递至关重要,调节基因转录. 在Drosophila Presenilin中,Null突变取消了Notch信号,防止其细胞内域的核进入.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 神经科学是一个神经科学.
背景情况:
- 普列尼林是膜蛋白,通过β-粉样蛋白前体蛋白处理与阿尔茨海默病的病原发生有关.
- 已知普列尼林可调节LIN-12/Notch跨膜受体的活性.
- 诺奇受体的细胞内域转移到细胞核,以控制带诱导的处理后的基因表达.
研究的目的:
- 为了研究多索菲拉普雷西尼林 (Drosophila Presenilin) 在Notch信号传导中的作用.
- 为了确定是否需要Presenilin用于Notch细胞内域的核转移.
- 阐明Presenilin影响Notch受体激活的机制.
主要方法:
- 在Drosophila Presenilin基因中利用了零突变.
- 评估的Notch信号传导路径.
- 检查了诺奇细胞内域的核入口.
- 研究了诺奇细胞内域的蛋白质分解释放.
主要成果:
- 在Drosophila Presenilin中发生的零突变完全取消了Notch信号传导.
- 素缺乏阻止了诺奇的细胞内域进入细胞核.
- 发现前列尼林对于从膜中诱导诺奇细胞内域的联体诱导的蛋白解释是必不可少的.
结论:
- 普雷西尼林在促进Notch信号传导方面发挥着关键作用.
- 诺奇细胞内域的蛋白质分解处理和随后的核转移取决于普雷西尼林的功能.
- 这些发现突显了普雷西尼林在通过Notch路径调节基因转录方面的重要性.
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相关概念视频
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...