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细胞外和细胞内腺生产的量化:了解跨膜度梯度的理解
A Deussen1, M Stappert, S Schäfer
1Institut für Physiologie, Medizinische Fakultät Carl Gustav Carus, TU Dresden, Germany.
Circulation
|April 20, 1999
概括
抑制腺素运输通过增强细胞外生产而增加血腺素,而不是通过增加细胞内水平. 在有氧条件下,心脏细胞充当腺沉,而不是来源.
科学领域:
- 心血管生理学心血管生理学
- 生物化学 生物化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 腺膜传输的抑制剂增加了血腺的度.
- 在输送抑制后血腺的增加的原因尚不清楚.
- 之前的假设表明细胞内腺水平高于间歇性腺水平.
研究的目的:
- 在膜运输被抑制时,调查血腺含量升高的来源.
- 为了测试该假设,即在氧气充足的条件下,细胞溶液腺的度超过了间歇度.
主要方法:
- 隔离的几内亚猪心脏被用来测量腺素释放和细胞质腺素度 (通过S-adenosylhomocysteine积累).
- 专门用于腺膜运输,脱氨酶和激酶的特定抑制剂被使用.
- 一个分布式的多分部模型被用于数据分析.
主要成果:
- 总心脏腺素的产量为2294 pmol. 时间 min-1.1. g-1,具有8%的细胞外生产.
- 细胞内代谢导致70.3%的细胞外腺素被吸收.
- 阻断膜运输增加了间歇性腺大约2.8倍,导致近最大的冠状动脉扩张.
结论:
- 细胞质量高于间歇性腺的假设被拒绝.
- 显著的细胞外腺生产发生在心脏中.
- 在充足氧气的条件下,帕伦基马细胞作为氨酸的沉而不是来源.
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