从细胞核RENT复合体中释放Cdc14蛋白酸酶的Tem1依赖性释放触发了脱离线分裂的过程
W Shou1, J H Seol, A Shevchenko
1Division of Biology, California Institute of Technology, Pasadena, California 91125, USA.
Cell
|April 29, 1999
概括
一种新型蛋白质,Net1,通过控制Cdc14的活性来调节发芽酵母中的线粒退出. 网1形成了RENT复合体,在核中将Cdc14隔离到无相.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 发芽酵母中的线粒离子退出受Tem1 GTPase和Cdc14蛋白酸酶等必需蛋白质的调节.
- 这些蛋白质对于降低循环林依赖的激酶活性至关重要,以确保适当的细胞分裂.
研究的目的:
- 为了确定芽酵母中线粒脱出的新型调节者.
- 阐明NET1基因及其编码蛋白在细胞周期控制中的功能.
主要方法:
- 一种新型突变 (net1-1) 的基因分析,该突变绕过了tem1删除的致死性.
- 质谱分析以确定RENT复合体的组件.
- 在细胞周期期间对RENT复合组件进行细胞下局部化研究.
主要成果:
- 网1-1突变绕过了 mitotic 出口中对Tem1的基本要求.
- NET1编码了一种新型蛋白质,该蛋白质是RENT复合体的关键组成部分,与Cdc14和Sir2.2一起.
- 该RENT复合物定位到从G1到亚纳相的核细胞,抑制Cdc14活性.
- 在晚期的亚纳相中,Cdc14以Tem1依赖的方式与RENT分离,从而启动了线粒体的退出.
结论:
- Net1通过RENT复合体将Cdc14隔离到细胞核内,从而起到抑制Cdc14的作用.
- 从RENT复合体释放的Cdc14依赖于Tem1的释放触发了线粒体的退出.
- 核细胞封存是调节各种生物过程的潜在通用机制.
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