Pch2将染色体沉默与介质检查点控制联系在一起
1Howard Hughes Medical Institute, and Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-8103, USA.
Cell
|May 13, 1999
概括
酵母中的PCH2基因对于介质检查点至关重要,防止染色体配对过程中的错误. 它的突变绕过了这种停止,导致染色体错误分离,并降低了子的活力.
科学领域:
- 细胞生物学 细胞生物学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 在Saccharomyces cerevisiae中的PCH2基因对于介质检查点至关重要.
- 这一检查点在重组和突触有缺陷时防止染色体分离.
研究的目的:
- 调查PCH2在介质检查点中的作用.
- 了解Pch2蛋白的定位和功能.
主要方法:
- 对具有PCH2突变的介质突变 (zip1,zip2,dmc1) 的分析.
- 蛋白质局部化研究 (细胞核,端粒).
- 调查声因子 (Sir2,Sir3) 的作用.
主要成果:
- 在突变者中,PCH2突变绕过了帕奇基因被捕,导致染色体错误分离.
- Pch2蛋白定位到核中,并抑制介质重组.
- Pch2的核定位取决于Sir2;Sir2突变也绕过了逮捕.
- Pch2还可以局部化到端粒,由Sir3介导,提供检查点功能.
结论:
- PCH2是介质性巴基检查点的关键组成部分.
- Pch2的功能与核细胞和端粒局部化有关.
- 发现连接了细胞核,染色体沉默和介质检查点.
相关概念视频
Epigenetic Regulation
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Position-effect Variegation
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Separation of Sister Chromatids
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The Spindle Assembly Checkpoint
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Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
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Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
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