体上的一种外套蛋白质,参与了真菌细菌的细胞内生存
概括
活生生的真菌细菌通过保留TACO蛋白在胞体上来阻止宿主防御. 这防止了 lysosomal 降解,允许病原体在巨细胞内存活.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 菌根菌是细胞内病原体,通过不太了解的机制,在巨细胞体内逃避宿主防御.
- 发酵体成熟,涉及与溶解体融合以降解,是主体防御的关键策略.
- 菌根细菌干扰菌体成熟的机制基本上是未知的.
研究的目的:
- 为了确定参与巨体内的真菌细菌生存的宿主因素.
- 阐明菌根细菌逃避 lysosomal 降解的机制.
- 研究宿主蛋白TACO在真菌细菌细胞内生存中的作用.
主要方法:
- 鉴定和表征一个宿主 WD重复蛋白 (TACO) 招募到虫体.
- 分析活菌与死菌的TACO招募和保留.
- 在有TACO表达和没有TACO表达的巨细胞中评估菌根的命运.
- 研究TACO对非巨细胞中的溶酶体输送的影响.
主要成果:
- 主体WD重复蛋白,TACO,被活生生的真菌细菌积极招募并保留在菌体上.
- TACO是通常在 lysosome 融合之前释放的 phagosome 层的组成部分.
- 缺乏TACO的巨细胞可以有效地将线粒细菌送到 lysosomes 中进行降解.
- 在非巨细胞中TACO的表达抑制了 lysosomal 的传递,并延长了菌根菌的存活时间.
结论:
- 活生生的菌根细菌在菌体上活性保留TACO是一种逃避宿主免疫力的新机制.
- 这种保留阻止了虫体-溶解体的融合,从而允许菌根菌在细胞内生存.
- 准TACO或其与真菌菌的相互作用可能代表对抗真菌菌感染的治疗策略.
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