Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Phagocytosis00:41

Phagocytosis

Cells pull particles inward and engulf them in spherical vesicles in an energy-requiring process called endocytosis. Phagocytosis (“cellular eating”) is one of three major types of endocytosis. Cells use phagocytosis to take in large objects—such as other cells (or their debris), bacteria, and even viruses.The objective of phagocytosis is often destruction. Cells use phagocytosis to eliminate unwelcome visitors, like pathogens (e.g., viruses and bacteria). It is perhaps unsurprising, that many...
What is Monogastric Digestion?01:50

What is Monogastric Digestion?

The human body contains a monogastric digestive system. In a monogastric digestive system, the stomach only contains one chamber in which it digests food. Several other animal species also have monogastric digestive systems, including pigs, horses, dogs, and birds. This chapter, however, focuses on the human digestive system.
The Angiosperm Life Cycle02:39

The Angiosperm Life Cycle

Plants have a life cycle split between two multicellular stages: a haploid stage—with cells containing one set of chromosomes—and a diploid stage—with cells containing two sets of chromosomes. The haploid stage is the gamete-producing gametophyte, and the diploid stage is the spore-producing sporophyte.
Phagocytosis00:41

Phagocytosis

Cells pull particles inward and engulf them in spherical vesicles in an energy-requiring process called endocytosis. Phagocytosis ("cellular eating") is one of three major types of endocytosis. Cells use phagocytosis to take in large objects, such as other cells (or their debris), bacteria, and even viruses.
The objective of phagocytosis is often destruction. Cells use phagocytosis to eliminate unwelcome visitors, like pathogens (e.g., viruses and bacteria). Many immune system cells, including...
Digestive Functions of the Large Intestine01:20

Digestive Functions of the Large Intestine

The large intestine is where the final stages of digestion happen. When the cecum receives chyme, it contains undigested carbohydrates that undergo fermentation. Gut bacteria ferment these carbohydrates to produce short-chain fatty acids that provide some energy and help synthesize essential vitamins.
As the chyme moves to the colon, it triggers two characteristic sluggish contractions - haustral churning and mass peristalsis. Haustral churning involves the rhythmic contraction and relaxation...
The Mammary Glands01:12

The Mammary Glands

The female breast is a hemispheric projection of variable size positioned anterior to the pectoralis major and serratus anterior muscles. A fascia layer composed of dense, irregular connective tissue connects it to these muscles.
Each breast features a pigmented projection known as the nipple, through which milk emerges via closely spaced openings of ducts, referred to as lactiferous ducts. Surrounding the nipple is a circular pigmented area of skin named the areola, which appears rough due to...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Nitric oxide is involved in the expression of neocortical spike-and-wave spindling episodes in DBA/2J mice.

The Journal of pharmacy and pharmacology·2003
Same author

Delayed presentation of congenital diaphragmatic Bochdalek hernia. Case report.

Minerva pediatrica·2003
Same author

Spectrum of SCN1A mutations in severe myoclonic epilepsy of infancy.

Neurology·2003
Same author

Family study of epilepsy in first degree relatives: data from the Italian Episcreen Study.

Seizure·2003
Same author

Alterations in smooth muscle contractile and cytoskeleton proteins and interstitial cells of Cajal in megacystis microcolon intestinal hypoperistalsis syndrome.

Journal of pediatric surgery·2003
Same author

Evidence for the presence of both peroxisome proliferator-activated receptors alpha and beta in the rat spinal cord.

Journal of chemical neuroanatomy·2003

相关实验视频

Updated: Jun 30, 2026

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
11:13

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Published on: December 1, 2015

哺乳动物的端粒以一个大双重循环结束.

J D Griffith1, L Comeau, S Rosenfield

  • 1Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill and Curriculum in Genetics and Molecular Biology, 27599-7295, USA. jdg@med.unc.edu

Cell
|May 25, 1999
PubMed
概括

端粒保护蛋白TRF2将端粒DNA重塑成大型双重环 (t环). 这些在人类和小鼠细胞中观察到的t循环,可以保护和复制染色体末端.

科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 哺乳动物的端粒由由蛋白质结合的重复性DNA序列组成,包括TRF1和TRF2.
  • TRF2对于端粒末端的保护至关重要;其抑制导致染色体末端的降低保护和融合.

研究的目的:

  • 研究TRF2在端粒维护中的结构性作用.
  • 为了阐明端粒末端保护的机制.

主要方法:

  • 使用电子显微镜进行体外生化分析,观察DNA结构变化.
  • 使用电子显微镜分析人类和小鼠细胞的psoralen交叉链接的端粒DNA.

主要成果:

  • 在实验室中,TRF2被证明可以将线性端粒DNA重塑为大型双重环 (t环).
  • 在人类和小鼠细胞的端粒DNA中发现了大量的t循环.
  • 有证据表明,t循环通过3'悬挂入侵双重端粒阵列而形成,涉及TRF1和单链结合蛋白.

结论:

  • 端粒重复结合因子2 (TRF2) 在形成端粒环 (t环) 中起着关键作用.
  • T环是哺乳动物细胞中保存的结构,可能作为端粒保护和复制的机制.

更多相关视频

Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes
09:59

Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes

Published on: June 3, 2017

Bovine Mammary Gland Biopsy Techniques
14:53

Bovine Mammary Gland Biopsy Techniques

Published on: December 23, 2018

相关实验视频

Last Updated: Jun 30, 2026

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
11:13

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Published on: December 1, 2015

Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes
09:59

Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes

Published on: June 3, 2017

Bovine Mammary Gland Biopsy Techniques
14:53

Bovine Mammary Gland Biopsy Techniques

Published on: December 23, 2018