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建立适用于治疗性血管生成的简单和实用的程序
1Department of Vascular Biology, Institute of Development, Aging, and Cancer, Tohoku University, Sendai, Japan.
Circulation
|May 25, 1999
概括
骨肌的电刺激通过增加血管内皮生长因子 (VEGF) 分泌来促进治疗性血管生成. 这种非侵入性方法可以增强缺血组织中的血液流动和毛细血管密度,而不会导致肌肉收缩或损伤.
科学领域:
- 生物医学工程 生物医学工程
- 再生医学是一种再生医学.
- 血管生物学 血管生物学
背景情况:
- 治疗性血管生成对于治疗缺血性疾病至关重要.
- 目前治疗血管生成的方法可能很复杂.
- 需要一种简单而实用的方法.
研究的目的:
- 开发一种用于治疗血管生成的简单而实用的程序.
- 为了研究非收缩性电刺激对血管生成的影响.
主要方法:
- 培养的骨肌细胞在非收缩性参数下受到电刺激.
- 测量了血管内皮生长因子 (VEGF) mRNA和蛋白质水平.
- 用条件介质评估内皮细胞生长.
- 用大鼠后肢缺血模型来评估体内疗效.
主要成果:
- 电刺激显著增加了骨肌细胞的VEGF mRNA和蛋白质分泌.
- 来自刺激细胞的条件介质促进了内皮细胞的生长.
- 在患有后肢缺血的老鼠中,5天的电刺激 (50 Hz,0.1 V) 增加了肌肉血液流动.
- 在刺激的缺血肌肉中,VEGF合成和毛细血管密度显著增加.
- 该程序被很好地容忍,没有观察到任何不良影响.
结论:
- 非收缩电刺激是一种简单而实用的治疗血管生成的方法.
- 这种技术有效地促进VEGF介导的血管化在缺血性四肢.
- 这些发现支持这种方法在缺血性疾病的临床应用.
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