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相关概念视频

Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...

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相关实验视频

Updated: Jul 10, 2026

Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method
09:54

Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method

Published on: December 16, 2014

肌细胞在心脏扩张性心肌病症中具有阳性DNA in situ末标记 (TUNEL) 的意义:不是细胞亡,而是DNA修复.

M Kanoh1, G Takemura, J Misao

  • 1Second Department of Internal Medicine, Gifu University School of Medicine, Gifu, Japan.

Circulation
|June 3, 1999
PubMed
概括

在扩张性心肌病 (DCM) 中,大多数通过TUNEL染色识别为亡的细胞实际上是经历DNA修复的活体肌细胞. 这一发现挑战了以前关于DCM中肌细胞细胞死亡的假设.

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Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover
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Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes
09:41

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

Published on: February 24, 2017

相关实验视频

Last Updated: Jul 10, 2026

Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method
09:54

Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method

Published on: December 16, 2014

Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover
08:03

Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover

Published on: May 23, 2016

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes
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Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

Published on: February 24, 2017

科学领域:

  • 心血管生物学 心血管生物学
  • 细胞病理学细胞病理学
  • 分子心脏病学分子心脏病学

背景情况:

  • 此前曾在患有扩张性心肌病 (DCM) 的人类心脏中使用DNA in situ nick end-labeling (TUNEL) 报告过亡性肌细胞.
  • 然而,缺乏明确的超结构性证据支持DCM中的肌细胞亡.

研究的目的:

  • 为了研究扩大心肌病中TUNEL阳性肌细胞的超结构特征.
  • 为了确定DCM中的TUNEL阳性肌细胞是否代表真正的细胞亡或替代细胞过程.

主要方法:

  • 来自20名DCM患者和20名对照组的内心肌瘤活检.
  • 光和电子显微镜与道染色 (EM-TUNEL).
  • 对于增殖细胞核抗原 (PCNA) 和Ki-67的DNA在位结合试验和免疫组织化学.

主要成果:

  • 在15%的DCM标本中发现了TUNEL阳性肌细胞,但在对照中没有发现.
  • 电子显微镜揭示了TUNEL阳性肌细胞具有奇怪的核与染色质凝聚,但它们的超结构不是apoptotic或necrotic,看起来像活细胞.
  • 现场DNA结合试验呈阴性,TUNEL阳性肌细胞表达PCNA但不表达Ki-67,排除了增殖.

结论:

  • 在DCM心脏中的大多数TUNEL阳性肌细胞没有经历细胞亡.
  • 这些细胞是活生生的肌细胞,表现出增加的DNA修复活性.