家庭盒基因Phox2b对于自主神经衍生物的发展至关重要
1Laboratoire de Génétique et Physiologie du Développement, Developmental Biology Institute of Marseille, CNRS/INSERM/Université de la Méditterranée/AP de Marseille, France.
Nature
|June 9, 1999
概括
宿主体转录因子Phox2b对于自主性腺节的发育至关重要. 它的缺失阻止了交感,副交感和肠道神经元的形成和存活.
科学领域:
- 神经科学是一个神经科学.
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
背景情况:
- 周围自主神经系统包括交感性,副交感性和肠道,所有这些都源自神经细胞.
- 自主性状腺发展的关键因素包括转录因子Mash1,状腺衍生神经营养因子 (GDNF) 和它的受体,以及神经调节蛋白信号传递.
研究的目的:
- 调查主体域转录因子Phox2b在自主性腺体发育和存活中的作用.
- 阐明Phox2b影响自主神经元分化和表型的分子机制.
主要方法:
- 对缺乏Phox2b的小鼠进行分析,以观察自主性腺的形成和生存.
- 基因表达分析关键发育因子 (Ret,Mash1) 和神经递质生物合成酶 (多巴胺-β-基酶,氨酸基酶) 在突变的关节原体中.
主要成果:
- 缺少Phox2b会导致所有自主性淋巴结的形成失败和退化,包括参与自主反射回路的头骨感官淋巴结.
- Phox2b对于GDNF受体子单元Ret的表达和在发育中的肠道和交感神经系统中维持Mash1表达至关重要.
- 突变的腺原体无法表达多巴胺β-基酶和氨酸基酶的基因,这表明Phox2b在调节诺亚类表型中的作用.
结论:
- 在脊椎动物中,Phox2b对于自主性淋巴细胞的适当发育,形成和存活是不可或缺的.
- Phox2b调节关键的分子通路,包括GDNF信号传递和Mash1表达,这对自主神经生成至关重要.
- Phox2b控制着在自主神经元中获得和维持诺亚类表型的过程.
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