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Updated: Jul 8, 2026

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通过高流动性组蛋白识别青改性DNA的基础

U M Ohndorf1, M A Rould, Q He

  • 1Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139, USA.

Nature
|June 29, 1999
PubMed
概括

西斯 (cis-diamminedichloroplatinum) 的抗癌活性涉及DNA损伤. 高流动性组 (HMG) 域蛋白与西斯胺改变的DNA结合,这是通过结构研究阐明的关键相互作用.

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Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...